乙型肝炎患者HBV M和HBV DNA的相关性研究

目的 探讨乙型肝炎患者的乙型肝炎病毒（ＨＢＶ）血清学标志（ＨＢＶ Ｍ）与ＨＢＶ ＤＮＡ检测结果的相关性与临床意义。方法 对４１４例乙型肝炎的ＨＢＶ Ｍ和ＨＢＶ ＤＮＡ检测结果进行比较。ＨＢＶ Ｍ用ＥＬＩＳＡ定量分析法检测，ＨＢＶ ＤＮＡ用斑点杂交法检测。结果 急性、慢性乙型肝炎患者中ＨＢＶ ＤＮＡ的阳性率与乙型肝炎肝硬化患者的ＨＢＶ ＤＮＡ阳性率比较，差异有显著性；ＨＢｓＡｇ、抗－ＨＢｅ、抗－ＨＢｃ阳性和ＨＢｓＡｇ、ＨＢｅＡｇ、抗－ＨＢｃ阳性组的ＨＢＶ ＤＮＡ阳性率比较，差异无显著性；ＨＢｓＡｇ和／或ＨＢｅＡｇ的滴度与ＨＢＶ ＤＮＡ阳性率呈正相关关系。结论 ＨＢＶ ＤＮＡ是评价ＨＢＶ活动最理想的标志；抗－ＨＢｅ的出现不能作为ＨＢＶ复制停止的指标；ＨＢｓＡｇ的滴度和ＨＢｅＡｇ的滴度变化可作为临床评价病毒复制程度和

Study of relativity between HBV M and HBV DNA in the cases with hepatitis B

Objective Serum samples of 414 cases with hepatitis B were detected for hepatitis B markers (HBVM) and HBV DNA,in order to investigation the relationship between HBV M and HBV DNA,and its clinical significant. Methods HBV M and HBV DNA were detected by ELISA and blot hybridization methods respectively.Results HBV DNA positive rates in the groups of acute and chronic hepatitis B were higher than that in the group of liver cirrhosis ( P < 0.01 ); HBV DNA positive rates had no apparent difference between the HBsAg,HBeAg, anti HBc positive group and the HBsAg, anti HBe,anti HBc positive group. Direct relativity was shown between the titer of HBsAg and/or HBeAg and the positive rate of HBV DNA. Conclusion HBV DNA can be treated as a sign of HBV activity; the appearance of anti HBe can not be taken as indicator of ceasing of HBV replication; the changes in HBsAg and HBeAg titers may be used as clinical evaluation on HBV replication and index for anti viral therapeutic effect.