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| 己烯雌酚人工抗原的合成及其抗体制备 | |
| 引用本文： | 邴爱英,成连贵,朱建华,牛钟相.己烯雌酚人工抗原的合成及其抗体制备[J].西北农业学报,2006,15(5):17-20. |
| 作者姓名： | 邴爱英 成连贵 朱建华 牛钟相 |
| 作者单位： | 1. 山东农业大学动物科技学院,泰安,271018 2. 滨州职业学院生物工程系,滨州,256624 3. 齐鲁动物保健品有限公司,济南,250100 |
| 摘 要： | 以己烯雌酚(DES)、琥珀酸酐和牛血清白蛋白(BSA)等为主要原料,采用混合酸酐反应(氯甲酸异丁酯法)将半抗原己烯雌酚与载体蛋白BSA联接制备成人工免疫原。用此免疫原免疫家兔,并以间接ELISA法测定抗体效价。结果表明,人工合成的抗原具有很强的免疫原性,被免疫兔血清中的抗DES抗体效价高达28。本试验为下一步制备抗DES单克隆抗体及研制快速检测DES残留的免疫试剂盒奠定了基础。 |
| 关 键 词： | 己烯雌酚 混合酸酐反应 人工抗原 抗体 |
| 文章编号： | 1004-1389（2006）05-0017-04 |
| 收稿时间： | 2005-12-26 |
| 修稿时间： | 2006-03-25 |
Synthesis of Artificial Antigen of Diethylstilbestrol and the Preparation of Its Antibody |
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| BING Ai-ying,CHENG Lian-gui,ZHU Jian-hua and NIU Zhong-xiang.Synthesis of Artificial Antigen of Diethylstilbestrol and the Preparation of Its Antibody[J].Acta Agriculturae Boreali-occidentalis Sinica,2006,15(5):17-20. | |
| Authors: | BING Ai-ying CHENG Lian-gui ZHU Jian-hua and NIU Zhong-xiang |
| Affiliation: | College of Animal Science and Technology, Shandong Agricultural University, Taian 271018, China;Department of Bioengineering, Binzhou Vocational College, Binzhou 256624, China;Qilu Animal Health Products Co., LTD, Ji''nan 250100, China;College of Animal Science and Technology, Shandong Agricultural University, Taian 271018, China |
| Abstract: | Using diethylstilbestrol(DES),succinic anhydride and bovine serum albumin(BSA) as main stuff,the artificial-immunogen has been obtained by the mixed acid anhydride reaction(isobutyl chloroformate method) to connect hapten DES with carrier-protein BSA.Six female rabbits were immunized subcutaneously with this artificial-immunogen,the serum value of antibody were measured with indirect ELISA test.Animal immune test demonstrated that there were antibody against DES in rabbits serum,and the value of the antibody was 2~8.This study laid the basis of further research on the immune test kit for the DES remnant and Monoclonal antibody against the DES. |
| Keywords: | Diethylstilbestrol Mixed acid anhydride reaction Artificial antigen Antibody |
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