Simultaneous HPLC quantification of total cholesterol,tocopherols and β-carotene in Barrosã-PDO veal |
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Authors: | José A Mestre Prates Mário A Gonçalves Quaresma Rui J Branquinho Bessa Carlos MG Andrade Fontes Cristina MP Mateus Alfaia |
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Affiliation: | 1. Department of Biochemistry, Faculty of Veterinary Medicine – CIISA, Rua Professor Cid dos Santos, Pólo Universitário do Alto da Ajuda, 1300-477 Lisboa, Portugal;2. Estação Zootécnica Nacional, Instituto Nacional de Investigação Agrária e das Pescas, Fonte Boa, 2005-048 Vale de Santarém, Portugal |
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Abstract: | A simple, rapid and sensitive procedure for the simultaneous determination of total cholesterol, tocopherols and β-carotene in meat is described. The method involves a direct saponification of the meat, a single n-hexane extraction and the analysis of the extracted compounds by normal-phase HPLC, using fluorescence (tocopherols) and UV–Vis photodiode array (cholesterol and β-carotene) detections in tandem. Rates of recovery of spiked meat samples were 93% for cholesterol, 83–86% for (α-, β- and -γ) tocopherols and 89% for β-carotene. Repeatabilities were high (CV < 6%) for all determined compounds, except for δ-tocopherol. This tocopherol, which is not usually present in meat, showed a much lower recovery percentage (73%) and repeatability (12.8%). This methodology was applied for the quantification of total cholesterol, tocopherols and β-carotene in three muscles (longissimus thoracis, longissimus lumborum and semitendinosus) of the Portuguese traditional Barrosã-PDO veal, obtained from autochthonous calves fed extensively during summer (with the least abundant green pastures) and slaughtered in early autumn (October). Barrosã-PDO veal showed median contents of total cholesterol (0.50–0.56 mg/g) and, depending on the analysed muscle, moderate to high contents of α-tocopherol (3.3–3.9 μg/g) and β-carotene (0.07–0.09 μg/g), suggesting an high sensorial and hygienic quality. |
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Keywords: | ANOVA analysis of variance BHT 2 4-di-tert-butyl hydroxytoluene CHR cholesterol β-CT β-carotene CV coefficient of variation HPLC high-performance liquid chromatography LL longissimus lumborum LT longissimus thoracis LSD least significant difference PDO protected designation of origin ST semitendinosus TF tocopherols UV ultraviolet |
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