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| 中国部分食品来源单增李斯特菌中致病相关基因的分布研究 | |
| 引用本文： | 贺春月,王艳,王天姝,叶正兴,代航,许柯,俞骅,汪永禄,张兰荣.中国部分食品来源单增李斯特菌中致病相关基因的分布研究[J].疾病监测,2013,28(5):384-388. |
| 作者姓名： | 贺春月 王艳 王天姝 叶正兴 代航 许柯 俞骅 汪永禄 张兰荣 |
| 作者单位： | 1. 贵阳医学院微生物学教研室,贵州贵阳550004;中国疾病预防控制中心传染病预防控制所,传染病预防控制国家重点实验室,北京昌平102206 2. 中国疾病预防控制中心传染病预防控制所,传染病预防控制国家重点实验室,北京昌平102206 3. 浙江省杭州市疾病预防控制中心 4. 安徽省马鞍山市疾病预防控制中心 5. 北京市通州区疾病预防控制中心 |
| 基金项目： | This study was supported by the National Science and Technology Key Project for Priority Communicable Diseases Prevention and Control，国家重大传染病防治专项课题 |
| 摘 要： | 目的 了解中国食品来源部分单增李斯特菌(Listeria monocytogences, LM)中致病相关基因的分布情况。 方法 383株LM分离自2002-2011年中国14省(市)的生肉、熟食、蔬菜、冷饮、水产等食品。采用PCR(polymerase chain reaction)和DNA打点杂交方法在单增李斯特菌中进行了55个致病相关基因的检测。 结果 50个致病相关基因检出率均为100%,inlF、fbpA和mprF基因检出率为99.73%,aut基因检出率为99.22%(380/383),vip基因检出率为98.17%(376/383)。 结论 383株食品来源单增李斯特菌中,大部分致病相关基因均存在,部分毒力基因的缺失存在多样性。 |
| 关 键 词： | 单增李斯特菌 致病相关基因 聚合酶链反应 DNA打点杂交 |
| 收稿时间： | 2013-01-15 |
Distribution of virulence-related genes of selected Listeria monocytogences strains isolated from food samples in China |
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| Abstract: | Objective To understand the distribution of virulence related genes of Listeria monocytogences strains isolated from food samples in China. Methods A total of 383 L. monocytogences strains were isolated from food samples, including raw meat, cooked food, vegetable, cold drink and aquatic product, in 14 provinces or municipalities. Polymerase chain reaction and DNA dot hybridization were conducted to detect 55 virulence related genes of L. monocytogences. Results The detection rates of the 50 virulence related genes were 100%, the detection rates of inlF, fbpA and mprF genes were 99.73%, the detection rate of aut gene was 99.22% and the detection rate of vip gene was 98.17%. Conclusion Most virulence related genes were detected in the 383 L. monocytogences strains from food samples in China, the deletion patterns of some virulence genes varied. |
| Keywords: | Listeria monocytogences virulence related gene polymerase chain reaction DNA dot hybridization |
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