A potential practical approach to reduce Ara h 6 allergenicity by gamma irradiation |
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| Authors: | Chunping Luo Chunqiu Hu Jinyan Gao Xin Li Zhihua Wu Anshu Yang Hongbing Chen |
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| Affiliation: | 1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China;2. Taizhou Vocational College of Science & Technology, Taizhou 318020, China;3. Department of Food Science, Nanchang University, Nanchang 330047, China;4. Department of Nutrition and Food Hygiene, Anhui Medical University, Hefei 230032, China;5. Sino-German Joint Research Institute, Nanchang University, Nanchang 330047, China;1. Institute of Agri-Food and Land Use, School of Biological Sciences, Queen''s University Belfast, Belfast, Northern Ireland, UK;2. Institute of Agri-Food and Land Use, ASSET centre, Queen''s University Belfast, Belfast, Northern Ireland, UK |
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| Abstract: | Peanut allergen Ara h 6 was isolated and irradiated at 1, 3, 5, or 10 kGy, and a whole peanut protein extract (WPPE) was also treated by irradiation. Alteration in structure of Ara h 6 was characterised by circular dichroism (CD) spectroscopy, ultraviolet (UV) absorption spectroscopy, fluorescence spectroscopy and SDS–PAGE, and antigenicity was evaluated by immunoblotting and indirect ELISA with anti-Ara h 6 polyclonal antibody. Irradiation induced significant changes in the secondary and tertiary structures of Ara h 6, and the antigenicity of both purified Ara h 6 and WPPE were reduced upon increasing the irradiation doses. Moreover, a good correlation between the loss in α-helix and IgG binding to Ara h 6 was observed. This indicated that irradiation might be an efficient approach to reduce or eliminate peanut allergenicity. |
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