| 不同采样期党参炔苷的含量动态变化分析 |
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| 引用本文： | 马琴国,安培坤,王引权.不同采样期党参炔苷的含量动态变化分析[J].甘肃中医,2014,0(2):23-25. |
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| 作者姓名： | 马琴国 安培坤 王引权 |
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| 作者单位： | 马琴国 (甘肃省中医院,甘肃 兰州,730050)； 安培坤 (甘肃省中医院,甘肃 兰州,730050)； 王引权 (甘肃中医学院)； |
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| 基金项目： | 甘肃省教育厅科研项目(项目编号：编号KY07-20) |
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| 摘 要： | 目的：分析不同采样期党参炔苷的含量。方法：炔苷含量采用高效液相色谱分析，TC-C18色谱柱（4.6 mm＆#215;250 mm，5μm），以乙腈-水（22∶78）为流动相,流速：1.0 mL/min。检测波长267 nm，柱温25℃。结果：党参炔苷在1.12～0.80μg/mL范围内呈良好的线性关系，r=0.9996，精密度RSD为1.28%（n=6），重复性RSD为1.9%，平均回收率为99.50%，RSD为1.90%（n=6），测定了不同采收期党参中党参炔苷的含量范围为0.87～3.00 mg/g。结论：分析了不同采样期党参炔苷的含量，为党参规范化栽培提供参考。
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| 关 键 词： | 不同采样期 党参 党参炔苷 高效液相 |
Dynamic Changes of the Contents,of Lobetyolin Contained in DangShen at Different Sampling Periods |
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| MA Qinguo,AN Peikun,WANG Yinquan.Dynamic Changes of the Contents,of Lobetyolin Contained in DangShen at Different Sampling Periods[J].Gansu Journal of Traditional Chinese Medicine,2014,0(2):23-25. |
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| Authors: | MA Qinguo AN Peikun WANG Yinquan |
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| Affiliation: | 1. Gansu Provincial Hospital of Traditional Chinese Medicine, Lanzhou 730050, China,. 2 Gansu University of Traditional Chinese Medicine) |
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| Abstract: | Objective：To analyze the contents of lobetyolin in DangShenCodonopsis pilosula（Franch.）Nannf.] at different sampling periods. Methods：The contents of lobetyolin were analyzed by HPLC, TC-C 18 chromatographic column （4.6 mm＆#215;250 mm, 5μm）, acetonitrile-water （22：78） were as the mobile phase, flow rate：1.0mL/min. Detec-tion wavelength was 267 nm, the temperature： 25℃. Results： Lobetyolin showed better linear relationship in the range between 1.12 to 0.80μg/mL, r=0.999 6, accuracy RSD was 1.28%（n=6）, repetition RSD was 1.9%, average recovery rate was 99.50%, RSD was 1.90% （n=9）, the contents of lobetyolin ranged from 0.87 to 3.00mg/g at different sampling periods.Conclusion：The contents of lobetyolin are analyzed in different sampling periods in order to provide reference for standardized cultivation of DangShen. |
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| Keywords: | different sampling periods DangShen lobetyolin HPLC |
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