| AFB1酶免试剂盒中酶标抗原的稳定性研究 |
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| 引用本文： | 龚燕,沈雯琰,赵春城,赵晓联.AFB1酶免试剂盒中酶标抗原的稳定性研究[J].安徽农业科学,2009,37(9):3882-3883. |
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| 作者姓名： | 龚燕 沈雯琰 赵春城 赵晓联 |
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| 作者单位： | 江苏省苏微微生物研究有限公司,江苏无锡,214063;江苏省苏微微生物研究有限公司,江苏无锡,214063;江苏省苏微微生物研究有限公司,江苏无锡,214063;江苏省苏微微生物研究有限公司,江苏无锡,214063 |
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| 摘 要： | 通过添加保护剂,使AFB1酶免试剂盒中冻干的酶标抗原溶解后能保存更长时间。采用4种保护剂（叠氮钠、硫柳汞、BSA、Proclin）分别加入酶标抗原稀释液进行ELISA试验,考察溶解状态下的酶标抗原保存在4℃冰箱和37℃保温箱中的稳定性。结果得出,加保护剂Proclin的稳定效果最好,酶标抗原溶液在4℃冰箱能保存6个月以上,提高了试剂盒的质量。
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| 关 键 词： | 保护剂 酶标抗原 稳定性 |
Research on the Stability of Enzyme-labelled Antigen in AFB_1 ELISA Kit |
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| GONG Yan et al.Research on the Stability of Enzyme-labelled Antigen in AFB_1 ELISA Kit[J].Journal of Anhui Agricultural Sciences,2009,37(9):3882-3883. |
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| Authors: | GONG Yan |
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| Affiliation: | GONG Yan et al(Jiangsu Suwei Microbiology Research Co.Ltd,Wuxi,Jiangsu 214063) |
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| Abstract: | The lyophilized enzyme-labeled antigen in AFB1 ELISA kit could be stored for a longer time by adding protective reagent.Four kinds of protective reagents(NaN3,Merthiolate,BSA and Proclin)were added to enzyme-labeled antigen buffer for ELISA test.The stability of dissolved enzyme-labeled antigen stored at 4 ℃ refrigerator and 37 ℃ incubator were observed.The results showed that the stability effect of Proclin was best.Enzyme-labelled antigen could be stored at 4 ℃ refrigerator for more than 6 months,which improved the quality of the kit. |
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| Keywords: | Protective reagent Enzyme-labelled antigen Stability |
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