高脂血对两种方法测定血红蛋白的影响及其校正

目的 观察高脂血对两种不同方法测定血红蛋白(Hb)的影响,并寻求校正高脂血干扰Hb测定的方法.方法 用Sysmex XE-5000全血细胞分析仪(十二烷基磺酸钠比色法,SLS-Hb法)及SIEMENS ADVIA2120全血细胞分析仪(氰化高铁Hb测定法,HiCN法)对30份高脂血标本进行全血细胞分析,低速离心后分别用等量生理盐水和仪器配套稀释液替代上层浑浊血浆,混匀后进行Hb测定,同时根据文献报道的Hb估算公式估算Hb值.结果 两种方法测定的直接测定组Hb、平均红细胞血红蛋白(MCH)、平均红细胞血红蛋白浓度(MCHC)均明显高于生理盐水组、稀释液组和估算组.结论 高脂血均造成两种方法测定Hb假性升高,生理盐水或稀释液等量置换血浆能消除高脂血对Hb测定的影响,可避免高脂血因素的影响,并能对Hb比色测定结果进行校正.

Influence of blood fat level on two methods for determination of hemoglobin and its correction

Objective To observe the influence of high level of blood fat on two different methods for determination of hemoglobin(Hb) and investigate method for rectifying the interferences of hyperlipidemia on the determination of hemoglobin.Methods 30 hyperlipidemia blood samples were analyzed for complete blood cell counts with Sysmex XE-5000i whole blood analyzer,based on sodium dodecylsulphate chromatometry,and SIEMENS ADVIA2120 whole blood analyzer,based on hemiglobincyanide method.The blood samples were centrifuged at low speed and the cloudy plasma was replaced by equal volume of normal saline and dilution solution of the instruments respectively.After mixture,detection of Hb was performed and Hb content was estimated according to reported estimation formula.Results Levels of Hb,mean corpuscular hemoglobin(MCH) and mean corpuscular hemoglobin concentration(MCHC),determined directly,were significantly higher than those,detected after different treatment or estimated.Conclusion Hyperlipoidemia might cause pseudo-increase of Hb level detected by the two methods.Replacement of plasma with equal volume of normal saline and dilution solution could eliminate and rectify the interference of hyperlipoidemia on the measurement of Hb.