An Improved Technique for the Rapid Chemical Characterisation of Bacterial Terpene Cyclases |
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| Authors: | Dr Jeroen S Dickschat Dr Khomaizon A K Pahirulzaman Patrick Rabe Tim A Klapschinski |
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| Affiliation: | Institut für Organische Chemie, TU Braunschweig, Hagenring 30, 38106 Braunschweig (Germany) |
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| Abstract: | A derivative of the pET28c(+) expression vector was constructed. It contains a yeast replication system (2μ origin of replication) and a yeast selectable marker (URA3), and can be used for gene cloning in yeast by efficient homologous recombination, and for heterologous expression in E. coli. The vector was used for the expression and chemical characterisation of three bacterial terpene cyclases. |
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| Keywords: | actinomycetes biosynthesis enzymes GC/MS heterologous expression terpene cyclases |
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