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疏水性石油烃降解菌的PCR测序及营养平衡研究
引用本文:赵晴,于鲁冀,张甲耀.疏水性石油烃降解菌的PCR测序及营养平衡研究[J].环境科学与技术,2009,32(1).
作者姓名:赵晴  于鲁冀  张甲耀
作者单位:1. 河南工业大学土木建筑学院,河南,郑州,450052
2. 郑州大学环境与水利学院,河南,郑州,450002
3. 武汉大学环境科学系,湖北,武汉,430079
摘    要:细胞表面疏水性是在研究细胞黏附物体表面领域最值得研究的细胞表面特性,研究表明其对疏水性有机物的去除率呈相关性。文章在对疏水性石油烃降解菌HDB-1生理生化特征、疏水能力及降解能力研究的基础上,继续对其16SrDNA序列进行分析,同时对其的营养平衡进行讨论。实验表明,PCR测序与细胞分类法得到的结果一致,菌株HDB-1属于假单胞菌属细菌(Pseudomonas),氮、磷则是影响HDB-1菌降解石油的限制因素。

关 键 词:PCR测序  营养平衡  疏水性降解菌

PCR Sequencing and Nutrient Balance of Hydrophobic Hydrocarbon Degrading Bacteria
ZHAO Qing,YU Lu-ji,ZHANG Jia-yao.PCR Sequencing and Nutrient Balance of Hydrophobic Hydrocarbon Degrading Bacteria[J].Environmental Science and Technology,2009,32(1).
Authors:ZHAO Qing  YU Lu-ji  ZHANG Jia-yao
Affiliation:1.School of Civil Engineering and Architecture;Henan University of Technology;Zhengzhou 450052;China;2.School of Environment and Water Conservancy;Zhengzhou University;Zhengzhou 450002;3.Department of Environment Science;Wuhan University;Wuhan 430079;China
Abstract:Cell-surface hydrophobicity as one of surface properties of the most worthy studied was related definitely with degradation of organic pollutant.Based on the study of physiological-biochemical characters as well as hydrophobic capability and degradation capability,the sequence of 16SrDNA and nutrient balance of hydrophobic hydrocarbon degrading bacteria named HDB-1were analyzed,experimental results showed that PCR sequencing was consistent with cell classification,and hydrophobicities of HDB-1 belong to Pse...
Keywords:PCR sequencing  nutrient balance  hydrophobic hydrocarbon degrading bacteria  
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