气相色谱法同时测定茶叶中10种有机磷农药残留

目的建立用气相色谱法同时测定茶叶中10种有机磷农药残留的方法。方法采用乙腈做萃取溶剂超声波提取,经CARB/NH2固相萃取小柱净化,用气相色谱法FPD检测器检测茶叶中10种有机磷农药。结果在0.05~1.0 mg/kg浓度范围内具有良好的线性关系,相关系数为0.999 63~0.999 86;在有机磷农药添加浓度为0.02~1.0 mg/kg,回收率80.0%~102.0%,相对标准偏差1.8%~5.8%,最低检出限(LOD,S/N=3)为0.002~0.013 mg/kg。结论建立的气相色谱法是一种操作简单、分离效果好、回收率高、方便快速的检测方法。

Simultaneously detection of 10 kinds of organophosphorus pesticides residues in tea by gas chromatography

A rapid, efficient and accurate method was developed for the determination of 10 kinds of organophosphorus pesticides in tea by gas chromatography.Methods 10 kinds of organophosphorus pesticides were extracted by ultrasonic assisted with acetonitrile as solvent, and next purified by CARB/NH₂ SPE cartridges. At last, they were detected by gas chromatography-FPD. Results The linear range of the method was 0.05-1.0 mg/kg (r=0.999 63-0.999 86), relative recoveries for the spiked samples with 0.02-1.0 mg/kg of organophosphorus pesticides were ranged from 80.0% to 102.0%; the relative standard deviations (RSD, n=5) was 1.8%-5.8%, and the limits of detection (S/N=3) was 0.002-0.013 mg/kg.Conclusion The proposed gas chromatography-FPD method is convenient, quick and easy maneuverability, high separation effective and recovery rate.