急性肺损伤模型大鼠核因子κB和细胞间黏附分子1表达与川芎嗪的干预

背景：研究表明,川芎嗪在抗肺纤维化方面发挥重要作用,但对川芎嗪抗急性肺损伤作用及其机制的研究较少。目的：观察大鼠油酸性急性肺损伤时,核因子κB和细胞间黏附分子1在肺组织中的表达及其变化。方法：采用静脉注射油酸的方法建立大鼠急性肺损伤模型,建模后并分别用川芎嗪和地塞米松进行干预。结果与结论：建模成功后,模型组大鼠肺系数明显升高（P〈0.05）。免疫组织化学染色结果显示,模型组肺组织核因子κB和细胞间黏附分子1的表达明显增强（P〈0.05）;应用川芎嗪和地塞米松干预后上述变化明显减轻（P〈0.05）。证实川芎嗪对急性肺损伤时的肺组织起到明显的保护作用,其保护机制与川芎嗪抑制核因子κB和细胞间黏附分子1的表达有关。

Effect of ligustrazine on nuclear factor kappa B and intercellular adhesion molecule-1 expression in a rat model of acute lung injury

BACKGROUND：Ligustrazine is quite effective in treating and preventing idiopathic pulmonary fibrosis（IPF）,but there are few reports describing the mechanism by which ligustrazine treats acute lung injury.OBJECTIVE：To observe the expression changes of nuclear factor-kappa B（NF-κB）and intercellular adhesion molecule-1（ICAM-1）in lung tissues of rats with acute lung injury induced by oleic acid.METHODS：Rat models of acute lung injury were established by intravenous injection of oleic acid and then interfered with ligustrazine and dexamethasone.RESULTS AND CONCLUSION：After successful induction of acute lung injury,the lung coefficient increased significantly in the model group compared with the control group（P 0.05）.Immunohistochemical staining results showed that NF-κB and ICAM-1 expression in the model group was significantly increased（P 0.05）.After ligustrazine and dexamethasone intervention,the above-mentioned changes were obviously alleviated（P 0.05）.These results suggest that ligustrazine exhibits an obvious protective effect on lung tissue during acute lung injury,and the mechanism might be related to the inhibition of NF-κB and ICAM-1 expression.