振动应力刺激骨髓间充质干细胞修复兔骨缺损

背景：研究证实,力学因素可调控、诱导骨髓间充质干细胞定向分化为骨细胞,提高分化效率。目的：观察振动应力刺激对兔骨缺损微环境中骨髓间充质干细胞移植修复肱骨骨缺损成骨分化能力的影响。方法：24只兔按随机数字表法分为非振动单纯骨基质明胶组、非振动骨基质明胶＋骨髓间充质干细胞复合植入组、振动骨基质明胶＋骨髓间充质干细胞复合植入组,每组8只,建立兔肱骨骨缺损模型。振动组兔置于振动平台,以0.3G的加速度,25Hz,正弦波型,1次/d,30min/次,持续4周施加振动刺激。结果与结论：造模4周后,大体观察结果显示,振动组骨痂生长良好,组织学切片显示其新生骨量较多,可见大量成骨细胞,骨缺损与断端形成骨性连接;振动组Ⅰ型胶原蛋白、RUNX2mRNA表达水平明显高于非振动组。提示振动应力刺激可促进骨缺损微环境中骨髓间充质干细胞的成骨分化能力,提高Ⅰ型胶原蛋白、RUNX2mRNA表达水平,从而加速骨缺损修复的进程。

Effect of vibration stress stimulation on bone marrow stem cells for repairing rabbit bone defects

BACKGROUND： Mechanical factors can regulate and induce the differentiation of bone marrow mesenchymal stem cells （BMSCs） into bone cells, and increase the differentiation efficiency. OBJECTIVE： To investigate the effect of vibration stress stimulation on the osteogenic differentiation ability of BMSCs in the bone defect microenvironment. METHODS： Twenty-four rabbits were randomized into three groups： bone matrix gelatin （BMG）, BMG＋BMSCs, vibration＋BMG＋BMSCs groups. Humeral bone defect models were established in all the rabbits. Vibration stress stimuli were applied to live animals with 0.3 G acceleration, 25 Hz, sine wave type, 1 once a day, 30 minutes once, for 4 weeks. RESULTS AND CONCLUSION： Gross observation display callus growth good in the vibration group, histological sections showed that the amount of new bone was large in the vibration group, and a large number of osteoblasts, bone formation and bone ends connected were found in the vibration group. mRNA expression of RUNX2 and type Ⅰ collagen was significantly higher in the vibration group than in the non vibration groups. Vibration can promote BMSCs, up-regulate RUNX2 gene, type Ⅰ collagen gene thus speeding up the repair of bone defects.