| 太阳鱼脑乙酰胆碱酯酶的分离纯化 |
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| 引用本文： | 王霞,刘铭钧,高俊娥,李盾.太阳鱼脑乙酰胆碱酯酶的分离纯化[J].应用与环境生物学报,2011(1):135-137. |
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| 作者姓名： | 王霞 刘铭钧 高俊娥 李盾 |
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| 作者单位： | 华南农业大学资源环境学院; |
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| 基金项目： | 广东省科技计划项目(No.2007B080401018)资助~~ |
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| 摘 要： | 采用硫酸铵沉淀–普鲁卡因胺亲和柱层析方法,对太阳鱼(Lepomis gibbosus)脑部乙酰胆碱酯酶分离纯化,纯化倍数为234倍,回收率为25.67%,经SDS-PAGE电泳检测,呈现单一的条带,相对分子质量约为68 000,表明其纯度达到电泳纯.
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| 关 键 词： | 乙酰胆碱酯酶 太阳鱼 纯化 亲和柱层析 |
Purification of Acetylcholinerase from Lepomis gibbosus Brain |
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| WANG Xia,LIU Mingjun,GAO Jun’e & LI Dun.Purification of Acetylcholinerase from Lepomis gibbosus Brain[J].Chinese Journal of Applied and Environmental Biology,2011(1):135-137. |
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| Authors: | WANG Xia LIU Mingjun GAO Jun’e & LI Dun |
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| Affiliation: | (College of Resources & Environment,South China Agricultural University,Guangzhou 510642,China) |
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| Abstract: | The acetylcholinesterase(AChE,EC 3.1.1.7) from sunfish(Lepomis gibbosus) brain was purified by ammonium sulfate and procainamide affinity chromatography.The purification fold reached 234 with activity recovery rate of 25.67%.By means of the SDS-PAGE analysis,the purified AChE preparation showed one band,and its relative molecular mass was about 68 000,which indicate the high degree of purification. |
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| Keywords: | acetylcholinesterase(AChE) Lepomis gibbosus(sunfish) purification affinity chromatography |
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