| 酶解法制备鹿茸胶原蛋白的工艺研究 |
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| 引用本文： | 徐云凤,赵雨,张鹤,李银清.酶解法制备鹿茸胶原蛋白的工艺研究[J].中华中医药杂志,2011(1):53-56. |
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| 作者姓名： | 徐云凤 赵雨 张鹤 李银清 |
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| 作者单位： | 长春中医药大学中医药与生物工程研发中心; |
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| 基金项目： | 国家“十一五”科技支撑计划项目(No.2007BAI38B06); 吉林省科技支撑计划项目(No.20096042)~~ |
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| 摘 要： | 目的:确定酶解法制备鹿茸胶原蛋白的最佳制备工艺。方法:用正交试验法考察胶原蛋白的提取工艺,用高效凝胶色谱法考察胶原蛋白的酶解条件,优选出鹿茸胶原蛋白提取的最佳工艺。结果:鹿茸胶原蛋白的制备工艺为:料液比(g:mL)1:3,水煮4h,提取5次,胰蛋白酶与底物(鲜鹿茸)的比是1:5 000,酶解时间为2h。结论:验证试验结果表明,鹿茸胶原蛋白收率为12.52%,蛋白含量为56.89%。本试验简便、稳定、可行。
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| 关 键 词： | 鹿茸 胶原蛋白 酶解 制备工艺 |
Study on preparation technology of collagen from Deer Antler by Trypsin hydrolysis |
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| XU Yun-feng,ZHAO Yu,ZHANG He,LI Yin-qing.Study on preparation technology of collagen from Deer Antler by Trypsin hydrolysis[J].China Journal of Traditional Chinese Medicine and Pharmacy,2011(1):53-56. |
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| Authors: | XU Yun-feng ZHAO Yu ZHANG He LI Yin-qing |
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| Affiliation: | XU Yun-feng,ZHAO Yu,ZHANG He,LI Yin-qing(Bio-engineering Lab,The Chinese Medicine and Bio-engineering Research Center of Changchun University of Chinese Medicine,Changchun 130117,China) |
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| Abstract: | Objective: To establish the optimal preparation technology of trypsin hydrolytic collagen from deer antler.Methods: Use orthogonal test to explore the extraction technology of collagen and explore the trypsin hydrolyze conditions by high performance liquid gel permeation chromatography,select the optimum preparation technology of trypsin hydrolytic collagen from deer antler.Results: The optimum preparation technology was obtained as follows: material-liquid rate(g:mL) was 1:3,boiled 4 hours,extracted five t... |
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| Keywords: | Deer Antler Collagen Trypsin hydrolysis Preparation technology |
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