| 氨基酸直接分析法原理及应用 |
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| 引用本文： | 牟世芬,丁永胜.氨基酸直接分析法原理及应用[J].现代科学仪器,2001(2):24-27. |
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| 作者姓名： | 牟世芬 丁永胜 |
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| 作者单位： | 中国科学院生态环境研究中心,Dionex 中国公司应用研究中心 |
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| 摘 要： | 本文介绍一种新型氨基酸分析方法-离子交换色谱-积分脉冲安培法（HPIC-IPAD），详细讨论方法的原理和应用，该方法与传统方法比较，无须进行柱后或柱前衍生反应，对氨基酸可以直接进行分析，灵敏度高，大多数氨基酸的最小检测限均小于1pmol，线性范围可以达到3个数量级以上。
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| 关 键 词： | 氨基酸 离子交换色谱－积分脉冲安培法 |
| 修稿时间： | 2001年4月16日 |
The Principle and Application of Amino Acids Analysis System |
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| Mou Shifen,Ding Yongsheng.The Principle and Application of Amino Acids Analysis System[J].Modern Scientific Instruments,2001(2):24-27. |
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| Authors: | Mou Shifen Ding Yongsheng |
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| Abstract: | A new method to analyze amino acids by ion exchange chromatography integrated pulsed amperometric detection is introduced. The principle and application of this method are discussed in detail. Comparing with traditional method, analysis of amino acids by this new method can be performed direct without post column and pre column derivatization. The new method has high sensitivity with the detection limit under 1 pmol and the linearity over three orders of magnitude for most common amino acids. |
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| Keywords: | Amino acids HPIC IPAD |
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