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| 以膨胀石墨为基底的聚吡咯电化学免疫传感器 | |
| 引用本文： | 陈智栋,杨艳伟,赵希岳,周民,王利群,孔泳.以膨胀石墨为基底的聚吡咯电化学免疫传感器[J].分析测试学报,2011,30(10):1123-1127. |
| 作者姓名： | 陈智栋 杨艳伟 赵希岳 周民 王利群 孔泳 |
| 作者单位： | 1. 常州大学,石油化工学院,江苏,常州,213164 2. 常州市第二人民医院,江苏,常州,213000 3. 常州大学,石油化工学院,江苏,常州,213164;浙江大学医学院附属第一医院,传染病诊治国家重点实验室,浙江,杭州,310003 |
| 基金项目： | 浙江大学医学院附属第一医院传染病诊治国家重点实验室开放基金 （2010KF03）；江苏省高校优势学科建设工程；江苏省自然科学基金资助项目（BK2009537） |
| 摘 要： | 用恒电位法在膨胀石墨基底表面合成聚吡咯,聚吡咯上的亚氨基与戊二醛发生交联,制备成稳定的膨胀石墨/聚吡咯/戊二醛传感器界面.以此界面固定人IgG抗体,戊二醛作为交联剂,发展了一种新型的电化学免疫传感器.该传感器在IgG溶液中温育后,其表面结合的IgG和随后加入的辣根过氧化氢酶(HRP)标IgG二抗以及传感器表面的IgG抗... |
| 关 键 词： | 膨胀石墨 聚吡咯 电化学免疫传感器 |
Polypyrrole Electrochemical Immunosensor Based on Expanded Graphite Substrate |
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| CHEN Zhi-dong,YANG Yan-wei,ZHAO Xi-yue,ZHOU Min,WANG Li-qun,KONG Yong.Polypyrrole Electrochemical Immunosensor Based on Expanded Graphite Substrate[J].Journal of Instrumental Analysis,2011,30(10):1123-1127. | |
| Authors: | CHEN Zhi-dong YANG Yan-wei ZHAO Xi-yue ZHOU Min WANG Li-qun KONG Yong |
| Affiliation: | CHEN Zhi-dong1,YANG Yan-wei1,ZHAO Xi-yue1,ZHOU Min3,WANG Li-qun1,KONG Yong1,2 (1.Institute of Petrochemical Technology,Changzhou University,Changzhou 213164,China,2.State Key Laboratory for Diagnosis and Treatment of Infectious Diseases,The First Affiliated Hospital of Medical College,Zhejiang University,Hangzhou 310003,3.The Second People's Hospital of Changzhou,Changzhou 213000,China) |
| Abstract: | Polypyrrole was synthesized on the surface of expanded graphite by potentiostatic electrodeposition.The imino group of polypyrrole combined with glutaraldehyde by crosslink to form a stable interface of expanded graphite/polypyrrole/glutaraldehyde.When human the IgG antibodies were immobilized onto the interface with glutaraldehyde as a cross linker,a novel electrochemical immonusensor was fabricated.The detection of human IgG is based on the principle of sandwich immunoassay.After the sensor was incubated ... |
| Keywords: | expanded graphite polypyrrole electrochemical immunosensor |
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