大鼠原位肝移植模型的建立

背景:"二袖套法"原位肝移植模型的建立缩短了无肝期的时间,并且显著提高了老鼠肝移植后的存活率。目的:在"二袖套法"的基础上,结合一些相关文献,建立稳定大鼠原位肝移植模型。方法:用改进的二袖套法对75对SD大鼠行原位肝移植,移植中门静脉、肝下下腔静脉用袖套法进行吻合,肝上下腔静脉用缝合法吻合,胆道采用支架法进行胆道重建。结果与结论:受体大鼠移植后一般状况良好,50例正式实验移植后1d存活率94%,1周存活率为90%。供肝热缺血时间均接近0,供体手术时间(34.44±3.25)min,受体手术时间(49.07±4.93)min,无肝期(17.26±2.51)min,下腔静脉平均阻断时间约为20min。说明只有熟练地掌握手术技巧,细致耐心的操作,最大程度减少各种并发症的发生,才能获得稳定的原位肝移植模型。

Establishing a rat model of orthotopic liver transplantation

BACKGROUND:Establishing a model of orthotopic liver transplantation by two-cuff technique can decrease the time without the liver and significantly increase the survival rate of the rats subjected to liver transplantation.OBJECTIVE:To establish the rats models of orthotopic liver transplantation based on two-cuff technique in combination with some related literatures.METHODS:Totally 150 Sprague Dawley rats were treated with liver transplantation by improved two-cuff method in the portal vein,hepatic inferior vena cava under the law in line with cuff,liver superior and inferior vena cava were sutured with suture line.Biliary tract reconstruction was completed in bile duct stent law.RESULTS AND CONCLUSION:Receptors rats were generally in good condition after operation.1-day survival rate was 94.0% and 1-week survival rate was 90.0% in 50 cases of the formal experiment.The time for donor operation and receptor operation was(34.44±3.25) minutes and(49.07±4.93) minutes,respectively,and anhepatic time was(17.26±2.51) minutes.The average blocking time of inferior vena cava was 20 minutes.To master the operation technique,operate patiently and carefully and reduce various complications can get a stable rat model of orthotopic liver transplantation.