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Stability of microencapsulated lactic acid bacteria under acidic and bile juice conditions
Authors:Dayana Pereira de Andrade  Cíntia Lacerda Ramos  Diego Alvarenga Botrel  Soraia Vilela Borges  Rosane Freitas Schwan  Disney Ribeiro Dias
Affiliation:1. Department of Biology, Federal University of Lavras, Lavras, MG, 37.200-000 Brazil;2. Department of Basic Science, Federal University of the Vales do Jequitinhonha and Mucuri, Diamantina, MG, 39.100-000 Brazil;3. Department of Food Science, Federal University of Lavras, Lavras, MG, 37.200-000 Brazil
Abstract:The probiotic strains Lactobacillus brevis CCMA1284 and Lactobacillus plantarum CCMA0359 were microencapsulated by spray drying using different matrices – whey powder (W), whey powder with inulin (WI) and whey powder with maltodextrin (WM). Viability of the microencapsulated strains in acid and bile juices and during 90 days of storage (seven and 25 °C) was evaluated. The two strains exhibited high encapsulation efficiency (> 86%) by spray drying. The different matrices maintained L. plantarum viability above six log CFU g?1 at 7 °C for 90 days, whereas similar results for L. brevis were observed only for W. The use of inulin as matrix of encapsulation did not enhance bacterial viability in the evaluated conditions. In general, the use of W and WM as matrices was effective for L. plantarum viability. However, only W was effective for L. brevis in the evaluated conditions. The spray drying technique was successfully adopted for the encapsulation of L. plantarum CCMA0359 and L. brevis CCMA1284 strains.
Keywords:Lactobacillus brevis  Lactobacillus plantarum  milk proteins  spray drying  viability
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