CLCN3/MMTV-PyMT转基因小鼠杂交群体的建立及鉴定

目的 建立并鉴定CLCN3/MMTV-PyMT双转基因小鼠杂交群体,构建氯通道ClC-3过表达的自发乳腺肿瘤转基因小鼠模型.方法 将CLCN3转基因小鼠和MMTV-PyMT转基因小鼠分别进行繁殖,选取阳性子代鼠进行配对,培育杂交后代,然后通过PCR鉴定基因型、通过组织免疫荧光方法、Western blot检测转基因小鼠蛋白表达情况.结果 成功繁育CLCN3转基因小鼠和MMTV-PyMT转基因小鼠,经PCR鉴定成功构建CLCN3/MMTV-PyMT双转基因鼠杂交群体,并成功保种和扩群,经组织免疫荧光和Western-blot分析双转基因小鼠的ClC-3蛋白的表达高于MMTV-PyMT转基因小鼠.结论 成功构建ClC-3过表达的自发乳腺肿瘤转基因小鼠模型,为进一步研究ClC-3在肿瘤生长和转移中的功能提供了良好的动物模型.

Establishment and identification of a ClC-3/MMTV-PyMT hybrid mouse strain

Objective To establish a CLCN3/MMTV-PyMT double transgenic mouse model of spontaneous breast tumor with simultaneously overexpressing ClC-3. Method CLCN3 transgenic mice were crossed with MMTV-PyMT spontaneous mammary tumor model mice. The genotype was determined by PCR. The expression of ClC-3 in tissues was detected by immunofluorescence and Western blot. Results CLCN3 and MMTV-PyMT transgenic mice were bred and CLCN3/MMTV-PyMT hybrid mouse model was successfully established. The ClC-3 expression in CLCN3/MMTV-PyMT hybrid mice was higher than that in the MMTV-PyMT mice, assessed by immunofluorescence and Western blot analysis. Conclusions Transgenic mouse models of spontaneous breast cancer with simultaneously overexpressing ClC-3 are successfully established. The double transgenic mice provide a good animal model for further research of ClC-3 in tumor growth and metastasis.