超声波-微波协同法提取分蘖葱头有机硫化合物的工艺优化及其组分分析

以分蘖葱头为试材,采用超声波-微波协同法提取有机硫化合物,进行分蘖葱头有机硫化合物工艺优化试验,并利用气相色谱-质谱联用仪对提取物组分进行分离鉴定,以期为进一步研究和开发分蘖葱头功能组分提供参考依据。结果表明:最佳提取工艺为微波温度55℃、料液比1∶8 g·mL^-1、协同时间6 min、搅拌转速300 r·min^-1、超声波功率500 W,该条件下有机硫化合物得率为0.2771%±0.0023%。该条件得到15种有机硫化合物,含量较高的是噻吩类39.0678%±0.0043%,其次是三硫化合物14.1570%±0.0071%和硫醚类7.3872%±0.0052%,其中含量相对较高的有机硫化合物为2,4-二甲基噻吩、3,5-二乙基-1,2,4-三硫杂环戊烷、二烯丙基二硫醚。

Optimization and Component Analysis of Ultrasonic-microwave Synergistic Extraction of Organic Sulfur-containing Compounds From Allium cepa L.var.agrogatum Don

Taking Allium cepa L.var.agrogatum Don as test material,organic sulfur-containing compounds were extracted by ultrasonic-microwave synergistic extraction.The process optimization test of organic sulfur compounds were carried out,and the components of extraction were separated and identified by gas chromatography-mass spectrometry in order to provide a reference for further research and development of functional components of Allium cepa L.var.agrogatum Don.The results showed that the optimal extraction process was the microwave temperature of 55 ℃,the ratio of material-liquid of 1∶8 g·mL^-1,the synergistic time of 6 minutes,the stirring speed of 300 r·min^-1,and the ultrasonic power of 500 W.The yield of the organic sulfur-containing compounds obtained under the above conditions was 0.277 1%±0.002 3%.Under this condition,fifteen kinds of organic sulfur-containing compounds were obtained.The higher content was thiophenes 39.067 8%±0.004 3%,followed by trisulfide compounds 14.157 0%±0.007 1% and thioethers 7.387 2%±0.005 2%.Among them,the relatively high content of organic sulfur-containing compounds was 2,4-dimethyl-thiophene,3,5-diethyl-1,2,4-trithiolane,diallyl disulphide.