| 长春地区人群人类疱疹病毒6型抗体的检测 |
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| 引用本文： | 黄红兰,李凡.长春地区人群人类疱疹病毒6型抗体的检测[J].中国免疫学杂志,2005,21(8):634-635. |
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| 作者姓名： | 黄红兰 李凡 |
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| 作者单位： | 吉林大学基础医学院病原生物学教研室,长春,130021 |
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| 基金项目： | 本课题受日本笸川医学研究助成金资助（2001） |
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| 摘 要： | 目的：建立检测血清中人类疱疹病毒6型（HHV-6）抗体的间接免疫荧光方法（IFA），检测人群中HHV-6抗体的水平。方法：用HHV-6GS株感染脐血单个核细胞制备抗原片，建立检测血清中HHV-6抗体的IFA法，并对长春市人群血清中的HHV-6抗体水平进行检测。结果：成功地建立了检测XHV-6抗体的间接免疫荧光方法，对长春市人群血清中的XHV-6抗体水平进行检测表明，XHV-6抗体阳性率为65．2％。结论：建立了特异性的IFA法，用于HHV-6感染的调查。
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| 关 键 词： | 人类疱疹病毒6型 抗体 间接免疫荧光试验 |
| 文章编号： | 1000-484X（2005）08-0634-02 |
| 收稿时间： | 2004-04-17 |
| 修稿时间： | 2004-04-17 |
Detection of human herpesvirus antibodies 6 in Changchun city |
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| HUANG Hong-lan,LI Fan.Detection of human herpesvirus antibodies 6 in Changchun city[J].Chinese Journal of Immunology,2005,21(8):634-635. |
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| Authors: | HUANG Hong-lan LI Fan |
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| Abstract: | Objective:To establish an indirect immunofluorescence assay for detection of serum IgG antibody against HHV-6,and to detected antibodies of HHV-6 in Changchun city.Methods:Cord blood mononuclear cells infected by HHV-6 strain GS were used to prepare cell antigen slide so as to establish an IFA and was used detect the HHV-6 antibody level in serum for Changchun city population.Results:An indirect immunofluorescence assay was established for detection of serum IgG antibody against HHV-6,at the same time the HHV-6 antibody level in serum for Changchun city population have been detected,and positive rate of HHV-6 antibody was 65.2%.Conclusion:A specific IFA has been established successfully for the investigation of HHV-6 infection. |
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| Keywords: | Human herpesvirus 6 Antibody Indirect immunofluorescence assay |
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