Short-chain fatty acids and polyamines in the pathogenesis of necrotizing enterocolitis: Kinetics aspects in gnotobiotic quails |
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| Authors: | AJ Waligora-Dupriet A Dugay N Auzeil I Nicolis S Rabot MR Huerre MJ Butel |
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| Affiliation: | 1. EA4065, Microbiologie, Faculté des sciences pharmaceutiques et biologiques, Université Paris Descartes, 4 avenue de l''Observatoire, 75006 Paris, France;2. Chimie Analytique, Faculté des sciences pharmaceutiques et biologiques, Université Paris Descartes, Paris, France;3. EA 2498 Biomathématiques, Faculté des sciences pharmaceutiques et biologiques, Université Paris Descartes, Paris, France;4. Unité d''Ecologie et Physiologie de Système Digestif (UEPSD), INRA, Jouy-en-Josas, France;5. Unité d''Histotechnologie et Pathologie, Institut Pasteur, Paris, France;1. Department of Neurology, Mayo Clinic, Rochester, Minnesota;2. Department of Medical Genetics, Mayo Clinic, Rochester, Minnesota;3. Department of Pediatrics, Mayo Clinic, Rochester, Minnesota;1. Université de Tunis El Manar, Faculté de Médecine de Tunis, CHU La Rabta, Laboratoire de Biochimie, LR99ES11, 1007 Jebbari, Tunis, Tunisia;2. Centre de Maternité et de Néonatologie de Tunis, Service de Néonatologie, 1007 Jebbari, Tunis, Tunisia;1. School of Animal Biology M085, Faculty of Science, The University of Western Australia, Crawley, Australia;2. The UWA Institute of Agriculture M082, The University of Western Australia, Crawley, Australia;3. Department of Poultry Science, University of Agriculture, Faisalabad, Pakistan;4. Department of Genetics and Animal Breeding, Warsaw University of Life Sciences, Warsaw, Poland;5. Avian Physiology and Genetics Division, Sálim Ali Centre for Ornithology and Natural History, Coimbatore, India;6. Department of Animal Sciences, University of Stellenbosch, Matieland, South Africa;1. Facultad de Medicina, Benemérita Universidad Autónoma de Puebla (BUAP), Puebla, Mexico;2. División Medicina Molecular, Centro de Investigación Biomédica de Occidente (CIBO), Instituto Mexicano del Seguro Social (IMSS), Guadalajara, Jalisco, Mexico;3. Multidisciplinary Research Group on Diabetes of the Instituto Mexicano del Seguro Social, Mexico, D.F., Mexico;4. Division of Endocrinology, Diabetes and Hypertension, Brigham and Women’s Hospital, Boston, Massachusetts;5. Laboratorio de Investigación en Fisiopatología de Enfermedades Crónicas, CIBIOR, IMSS, Atlixco, Puebla, Mexico |
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| Abstract: | Despite years of investigation, pathogenesis of necrotizing enterocolitis (NEC) remains elusive. Bacterial metabolites were implicated by several authors but their roles remain controversial. The aim of our study was to investigate the role of SCFAs and polyamines through a kinetic study of histological and macroscopical digestive lesions in monobiotic quails. Germ-free quails, inoculated with a Clostridium butyricum strain involved in a NEC case, were fed or not with a diet including lactose (7%). Quails were sacrificed at various times between D7 and D24 after bacterial inoculation. NEC-like lesions, i.e. thickening, pneumatosis, and hemorrhages, occurred only in lactose-fed quails and increased with time. The main histological characteristics were infiltrates of mononuclear cells, then heterophilic cells, then gas cyst and necrosis. The first event observed, before histological and macroscopical lesions, is a high production of butyric acid, which precedes an increase of iNOS gene expression. No difference in polyamines contents depending on the diet was observed. These results show the major role of butyric acid produced by commensal bacteria in the onset of the digestive lesions. |
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| Keywords: | Necrotizing enterocolitis Clostridium Short-chain fatty acid Nitric oxide synthase Gnotobiotic animal |
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