| RP-HPLC测定木通中木通苯乙醇苷B的含量 |
| |
| 引用本文： | 高慧敏,王智民 曲莉,付雪涛,李琳.RP-HPLC测定木通中木通苯乙醇苷B的含量[J].中国中药杂志,2007,32(6):476-478. |
| |
| 作者姓名： | 高慧敏 王智民 曲莉 付雪涛 李琳 |
| |
| 作者单位： | 中国中医科学院,中药研究所,北京,100700 |
| |
| 基金项目： | 国家高技术研究发展计划(863计划) |
| |
| 摘 要： | 目的：建立木通药材中木通苯乙醇苷B的HPLC分析方法。方法：采用Phenomenex Luna C18(4.6 mm×250 mm,5 μm)柱,以甲醇-水-磷酸(35∶65∶0.05)为流动相,流速1.0 mL·min-1,检测波长330 nm,柱温35 ℃。结果：木通苯乙醇苷B在0.04～0.86 μg呈良好的线性关系,r=0.999 8,平均回收率97.2%。对3个基源(三叶木通、五叶木通、白木通)的木通药材及其他部位(根、叶、果实)共39个样品进行了含量测定,各样品含量在0.021%～0.745%。结论：方法简单,重复性好,适合用于木通药材的质量控制。
|
| 关 键 词： | 木通 木通苯乙醇苷B |
| 文章编号： | 1001-5302（2007）06-0476-03 |
| 收稿时间： | 2006-01-19 |
| 修稿时间： | 2006-01-19 |
Determination of calceolarioside B in Caulis Akebiae by RP-HPLC |
| |
| GAO Hui-min; WANG Zhi-min; QU LI; FU Xue-tao; LI Lin.Determination of calceolarioside B in Caulis Akebiae by RP-HPLC[J].China Journal of Chinese Materia Medica,2007,32(6):476-478. |
| |
| Authors: | GAO Hui-min; WANG Zhi-min; QU LI; FU Xue-tao; LI Lin |
| |
| Affiliation: | Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. |
| |
| Abstract: | OBJECTIVE: To establish a quantitative method for calceolarioside B in Caulis Akebiae. METHOD: The samples were separated at 35 degrees C on a Phenomenex Luna C18 column eluted with methanol-water-phosphoric acid (35: 65: 0.05) as mobile phase. Flow rate was at 1.0 mL x min(-1) and the detection wavelength was at 330 nm. RESULT: The calibration curve was linear over the range from 0. 04 to 0. 86 g (r = 0. 9998) and the average recovery was 97.2%. 39 Crude drug materials collected from different areas were determined and the contents of calceolarioside B in Caulis Akebiae were fluctuated from 0. 021% to 0. 745%. CONCLUSION: The method is simple and repeatable and could be used for the quality control of Caulis Akebiae. |
| |
| Keywords: | HPLC |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
