| 改良自乳化溶剂扩散法制备MePEG-PLA纳米粒对成骨细胞的毒性 |
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| 引用本文： | 王忠磊,高岩,赖春花,卢海宾,李少冰,蒋颖,韦丛云,周磊.改良自乳化溶剂扩散法制备MePEG-PLA纳米粒对成骨细胞的毒性[J].中国组织工程研究与临床康复,2011,15(21):3851-3854. |
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| 作者姓名： | 王忠磊 高岩 赖春花 卢海宾 李少冰 蒋颖 韦丛云 周磊 |
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| 作者单位： | 南方医科大学附属口腔医院,广东省口腔医院,广东省广州市,510280 |
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| 基金项目： | the,National,Science,&,Technology,Pillar,Program,during,the,Eleventh,Five-Year,Plan,Period,No.2007BA,I18BO6 |
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| 摘 要： | 背景:两亲性嵌段聚合物由于其较强的载药能力强、纳米级大小、血液中长循环等优点在载药系统中得到广泛的应用。目的:评估改良自乳化溶剂扩散法制备的甲氧基封端的聚乙二醇-聚乳酸(MePEG-PLA)纳米粒对人骨肉瘤细胞MG63的毒性。方法:通过改良自乳化溶剂扩散法制备MePEG-PLA纳米粒,MTS法测定纳米粒培养1,2,3d后对MG63的毒性。激光粒度分析仪测定纳米颗粒的粒径大小、粒径分布及Zeta电位;透射电镜表征纳米胶束外观形态;酶标仪检测培养1,2,3d细胞吸光度值。结果与结论:MePEG-PLA纳米粒的平均粒径为25.7nm,分布均匀,呈球形,Zeta电位为-8.06mV,MePEG-PLA毒性为0级。提示改良自乳化溶剂扩散法制备纳米粒简单易行,制备的纳米粒无毒,具有良好的应用前景。
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| 关 键 词： | 纳米粒 药物载体 聚乳酸 聚乙二醇 改良自乳化溶剂扩散法 |
Cytotoxicity effect of MePEG-PLA nanoparticles prepared by modified self-emulsion solvent diffusion technique on osteoblasts in vitro |
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| Wang Zhong-lei,Gao Yan,Lai Chun-hua,Lu Hai-bin,Li Shao-bing,Jiang Ying,Wei Cong-yun,Zhou Lei.Cytotoxicity effect of MePEG-PLA nanoparticles prepared by modified self-emulsion solvent diffusion technique on osteoblasts in vitro[J].Journal of Clinical Rehabilitative Tissue Engineering Research,2011,15(21):3851-3854. |
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| Authors: | Wang Zhong-lei Gao Yan Lai Chun-hua Lu Hai-bin Li Shao-bing Jiang Ying Wei Cong-yun Zhou Lei |
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| Affiliation: | Wang Zhong-lei,Gao Yan,Lai Chun-hua,Lu Hai-bin,Li Shao-bing,Jiang Ying,Wei Cong-yun,Zhou LeiThe Affiliated Stomatological Hospital of Southern Medical University & Guangdong Provincial Stomatological Hospital,Guangzhou 510280,Guangdong Province,China |
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| Abstract: | BACKGROUND:Amphiphilic block copolymers have been widely applied in the drug delivery system because of the high capacity of drug carriers,nanoparticles and long circulation time.OBJECTIVE:To evaluate the cytotoxicity effect of MePEG-PLA nanoparticles prepared by modified self-emulsion solvent diffusion technique on MG63 in vitro.METHODS:MePEG-PLA nanoparticles were prepared by modified self-emulsion solvent diffusion technique.The cytotoxicity effect of MePEG-PLA nanoparticles on MG63 was detected using MT... |
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