| 灯盏乙素抑制LPS诱导BV2细胞株炎症细胞因子表达的研究 |
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| 引用本文： | 毛峻琴,孟慧,赵丹,王闰,郑悦.灯盏乙素抑制LPS诱导BV2细胞株炎症细胞因子表达的研究[J].第二军医大学学报,2011,32(11):1235-1238. |
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| 作者姓名： | 毛峻琴 孟慧 赵丹 王闰 郑悦 |
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| 作者单位： | 1. 解放军85医院药剂科,上海,200052
2. 第二军医大学药学院药理学教研室,上海,200433 |
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| 摘 要： | 目的研究灯盏乙素对脂多糖(lipopolysaccharide,LPS)诱导BV2细胞株炎症细胞因子表达的影响,探讨灯盏乙素抗炎作用机制。方法通过荧光素酶报告基因检测核转录因子NF-κB活性,并利用LPS刺激使BV2细胞和通过RT-PCR和蛋白免疫法检测TNF-α和IL-1β的mRNA及蛋白含量,研究灯盏乙素的抗炎作用。实验分为正常组、LPS刺激(1μg/ml)的模型组、灯盏乙素组(0.1、1、10和100μg/ml)。结果灯盏乙素预处理(1～100μg/ml)可抑制LPS引起pNF-κB 293的核转录因子NF-κB激活(P<0.05或P<0.01);灯盏乙素预处理(1～100μg/ml)能够抑制LPS引起BV2的TNF-α和IL-1βmRNA和蛋白含量升高(P<0.01)。结论灯盏乙素具有抗炎作用,其抗炎作用与抑制核转录因子NF-κB、抑制炎症细胞因子表达有关。
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| 关 键 词： | 灯盏乙素 炎症 细胞因子类 肿瘤坏死因子 白介素 |
| 收稿时间： | 6/8/2011 12:00:00 AM |
| 修稿时间： | 2011/10/10 0:00:00 |
Scutellarin inhibits LPS-induced pro-inflammatory cytokine expression in BV2 cells |
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| MAO Jun-qin,MENG Hui,ZHAO Dan,WANG Run and ZHENG Yue.Scutellarin inhibits LPS-induced pro-inflammatory cytokine expression in BV2 cells[J].Academic Journal of Second Military Medical University,2011,32(11):1235-1238. |
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| Authors: | MAO Jun-qin MENG Hui ZHAO Dan WANG Run and ZHENG Yue |
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| Affiliation: | 1. Department of Pharmacy, No.85 Hospital of PLA, Shanghai 200052, China;2. Department of Pharmacology, School of Pharmacy, Second Military Medical University, Shanghai 200433, China;1. Department of Pharmacy, No.85 Hospital of PLA, Shanghai 200052, China;2. Department of Pharmacology, School of Pharmacy, Second Military Medical University, Shanghai 200433, China;1. Department of Pharmacy, No.85 Hospital of PLA, Shanghai 200052, China;2. Department of Pharmacology, School of Pharmacy, Second Military Medical University, Shanghai 200433, China;1. Department of Pharmacy, No.85 Hospital of PLA, Shanghai 200052, China;2. Department of Pharmacology, School of Pharmacy, Second Military Medical University, Shanghai 200433, China;1. Department of Pharmacy, No.85 Hospital of PLA, Shanghai 200052, China;2. Department of Pharmacology, School of Pharmacy, Second Military Medical University, Shanghai 200433, China |
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| Abstract: | |
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| Keywords: | scutellarin inflammation cytokines tumor necrosis factor interleukin |
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