肺结核患者PBMC膜白介素-2受体(CD25)表达及其临床意义

目的:探讨外周血单个核细胞(PBMC)膜白介素-2受体(CD25)表达在肺结核病鉴别诊断中的应用价值.方法:用生物素-链霉亲和素(BSA)法检测肺结核、支气管肺炎患者T细胞亚群及植物血凝素(PHA)诱导前后CD25表达水平.结果:支气管肺炎患者CD3+、CD4+、CD8+水平分别为(62.32±6.34)%、(47.52±7.16)%、(32.12±6.55)%,CD4+/CD8+ 比值为1.52±0.43,PHA诱导前后CD25水平分别为(4.56±1.52)%、(35.12±7.21)%.空洞型肺结核CD3+、CD4+、CD8+、CD4+/CD8+水平分别为(41.13±5.25)%、(43.38±5.15)%、(36.25±3.46)%和1.15±0.21,非空洞型肺结核CD3+、CD4+、CD8+、CD4+/CD8+水平分别为(46.29±5.60)%、(47.21±4.86)%、(32.36±4.03)%、1.46±0.25,相互比较CD3+、CD4+/CD8+差异均有显著性(P＜0.01和P＜0.05).空洞型肺结核与非空洞型肺结核患者PHA诱导前后CD25水平分别为(2.13±1.14)%、(27.25±3.50)%和(3.43±1.35)%、(31.14±4.11)%,两者相比差异均有显著性(P＜0.01).结论:肺结核病患者体内存在明显的细胞免疫功能紊乱,主要表现为CD25表达水平降低,CD25表达水平与肺结核病的病情似有一定关系,其对肺结核病鉴别诊断具有重要价值.

The expression of membrane interleukin-2 receptor(CD25) on the surface of peripheral blood mononuclear cells(PBMC) of pulmonary tuberculosis and its clinical significance

Objective:To study the value of membrane interleukin-2 receptor(CD25) of peripheral blood mononuclear cells (PBMC) on the differential diagnosis of pulmonary tuberculosis. Methods:The expression of T cell subset and levels of CD25 before and after induction with PHA were detected by biotin-streptavidin(BSA) in patients with pulmonary tuberculosis and bronchopneumonia. Results:The levels of CD3+, CD4+, CD8+, CD4+/CD8+ and CD25 before and after induction in peripheral blood in patients with bronchopneumonia were(62.32±6.34)%,(47.52±7.16)%,(32.12±6.55)%, 1.52±0.43,(4.56±1.52)%,and (35.12±7.21)%,respectively. The levels in pulmonary tuberculosis with cavity were(41.13±5.25)%,(43.38±5.15)%,(36.25±3.46)%, 1.15±0.21,(2.13±1.14)%,and (27.25±3.50)% and in pulmonary tuberculosis without cavity were(46.29±5.60)%,(47.21±4.86)%, (32.36±4.03)%, 1.46±0.25,(3.43±1.35)%,and (31.14±4.11)%,respectively. The difference of CD3+, CD4+/CD8+ in patients with pulmonary tuberculosis and bronchopneumonia were significant(P＜0.01 and P＜0.05).Conclusions:The cellular immunity in patients with pulmonary tuberculosis is obviously in disorder, manifest mainly in lowering level of CD25. The level of CD25 seems to be associated closely with condition of patients. The detection of CD25 plays an importment role in diagnosis of pulmonary tuberculosis.