梯度洗脱高效液相色谱法测定不同剂型消毒剂中醋酸氯己定的含量

目的建立不同剂型消毒剂中醋酸氯己定含量的梯度洗脱高效液相色谱分析方法。方法以1份乙酸水+4份乙醇组成的提取液稀释处理样品,用Waters2695型高效液相色谱仪对不同样品进行检测。结果采用ZOR-BAX Eclipse XDB-C18色谱柱,用磷酸氢二钾+乙腈作为流动相,流量1.0 ml/min,检测波长260 nm为检测条件。该方法消毒剂中醋酸氯己定检出限为2.5 ng,回收率为96.1%～99.0%,线性范围10～300μg/ml,相关系数为0.9999。结论梯度洗脱高效液相色谱法测定醋酸氯己定的含量具有灵敏度高,线形范围宽,抗干扰能力强,适用于不同剂型消毒剂中醋酸氯己定的测定。

DETERMINATION OF CHLORHEXIDINE ACETATE IN DIFFERENT FORM OF DISINFECTANTS BY GRADIENT ELUTION HPLC SYSTEM

Objective To establish a gradient elution HPLC method for the determination of chlorhexidine acetate in different form of disinfectants.Methods The samples pretreated with extracting solution consisted of acetic acid solution and ethanol(1+4) were detected by Waters 2695 HPLC system.Results The HPLC system was consisted of a ZORBAX Eclipse XDB-C18 column.Acetonitril and phosphate buffer were used as the mobile phase at the rate of 1.0 ml/min and UV detection wave length was 260 nm.The detection limit for chlorhexidine acetate was 2.5 ng and the coverage ranged from 96.1% to 99.0%.The peak areas were linear to the chlorhexidine acetate in the range of 10～300 g/ml with the correlation coefficient r was 0.9999.Conclusion The method has high sensitivity,wide range for liner and strong anti-jamming ability and can be applied to the determination of chlorhexidine acetate in different form of disinfectants with satisfactory results.