纳洛酮对大鼠中重度创伤性脑损伤后细胞免疫功能影响的实验研究

目的：通过观察大鼠创伤性脑损伤（traumatic brain injury,TBI）后纳洛酮治疗前后血浆β-EP、CD4＋、CD8＋和IL-2的动态变化,探讨纳洛酮对大鼠TBI后细胞免疫功能变化的影响及作用机制。方法：采用气体冲击致大鼠中重度脑损伤模型;流式细胞术、RIA、ELISA检测血液中CD4＋、CD8＋、β-EP、IL-2的变化。结果：TBI大鼠经纳洛酮治疗后,血浆中CD4＋和IL-2含量升高,β-EP和CD8＋则降低。大、小剂量纳洛酮治疗在相应时间点比较有显著性差异（P〈0.05）。结论：纳洛酮可通过降低TBI大鼠血浆中β-EP,调节TBI后应激紊乱,竞争性抑制β-EP对免疫细胞受体的作用,从而使CD4＋、CD8＋和IL-2水平趋于正常,恢复免疫平衡,起到治疗与保护作用。

Experimental investigation on effect of cell immunity after naloxone hydrochloride treated severe traumatic brain injury in rats

Objective：To demonstrate the effects and potential mechanisms of cell immunity on rats with TBI by observing the changes of β-EP,CD^＋4,CD^＋8 and IL-2 in serum after the treatment of naloxone.Methods：（1）The models of severe brain injury were produced by gas percussion.（2） The levels of CD^＋4 and CD^＋8 in serum of rats were detected by Flow cytometer（FCM）;The levels of β-EP in serum of rats were detected by radioimmunoassay（RIA）;The levels of IL-2 in serum of rats were detected by Enzyme-linked immunoabsorbent Assay（ELISA）.Results：CD^＋4 and IL-2 in serum increased,while β-EP and CD^＋8 in serum decreased after treatment with naloxone.Compared to the curative effect of the low dosage nalxone and the high dosage naloxone,it is significantly different（P〈0.05）.Conclusions： Naloxone showed protective effects by degrading the concentration of β-EP and adjusting post-trauma stress disorder to regulate the levels of CD^＋4,CD^＋8,IL-2,and relieved the second brain injury and inhibition of cellular immunity caused by the disorder of immune balance in rats of TBI.