人脑胶质瘤中cyclin B1的表达及其与细胞增殖活性的关系

目的研究人脑胶质细胞瘤中细胞周期素B1（cyclin B1）的表达水平。及其胶质瘤细胞增殖活性的关系。方法采用SABC免疫组化方法检测62例人脑胶质瘤和14例正常脑组织标本中eyclin B1和增殖细胞核抗原（PCNA）的表达水平，观察并对比各病理等级中表达水平。结果cyclin B1在正常脑组织中表达率为7.1％（1／14），其阳性率和平均标记指数随着胶质瘤病理分级的增高而明显升高（P〈0.05）。双元相关性分析发现cyclin B1与PCNA的表达呈显著正相关（Pearson相关系数r=0.576，P〈0.01）。结论人脑胶质瘤中cyclin B1的表达与病理学分级和肿瘤细胞增殖活性关系密切，可能是肿瘤恶性增殖的促进因素。

Expression of Cyclin B1 and Its Relationship with Cellular Proliferation in Hu-man Brain Glioma

Objective To study the expression of cyclin B1 in human gliomas and its relationship with cellular proliferation activity. Methods The expression of eyclin B1 and PCNA were detected by immunohistochemistry in 62 human brain gliomas and 14 normal brain tissues in each WHO grade of human brain ghomas. Results Positive rate of cyclin B1 in normal brain tissues was 7.1% （ 1/ 14）. In human brain gliomas, positive rates and mean labeling indices for cyclin B1 increased with the advancing of pathological grades （P 〈 0.05 ）. Positive correlation was obtained between cyelin B1 and PCNA by bivariate correlation analysis （Pearson corre-lation coefficient r = 0.576, P 〈 0.01）. Conclusion Expression of cyclin B1 is correlated with the cellular proliferation activity as well as pathological grades in human brain glioma. It may be an irhportant factor in promoting the cell transformation to malignant phenotype.