| 基于iTRAQ技术的WB-F344细胞向胆管细胞分化过程中的蛋白质组学研究 |
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| 引用本文： | 唐庆贺,徐峰,李爱军,谈冶雄,周伟平.基于iTRAQ技术的WB-F344细胞向胆管细胞分化过程中的蛋白质组学研究[J].肝胆外科杂志,2011,19(6):455-458. |
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| 作者姓名： | 唐庆贺 徐峰 李爱军 谈冶雄 周伟平 |
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| 作者单位： | 第二军医大学东方肝胆外科医院,上海,200438 |
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| 摘 要： | 目的 应用iTRAQ技术观察WB-F344细胞向胆管细胞分化过程中蛋白质表达组的变化.方法 将WB-F344细胞接种于丁酸钠分化体系中进行分化诱导,提取不同时间点(第0、2、4、6天)的分化蛋白以iTRAQ试剂标记后进行质谱检测并以软件分析差异表达的蛋白质.结果 iRAQ试剂标记的WB-F344分化细胞蛋白质表达谱分析...
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| 关 键 词： | WB-F344细胞系 分化 蛋白质组学iTRAQ |
PROTEOME ANALYSIS IN BILIARY DIFFERENTIATION OF WB-F344 CELLS BY ITRAQ TECHNOLOGY |
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| Affiliation: | TANG Qing-he,XU Feng,LI Ai-jun,et al.Eastern Hepatobiliary Surgery Hospital,Shanghai 200438,China |
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| Abstract: | Objective To screen for the differentially expressed proteins during WB-F344 cells biliary differentiation by iTRAQ technique.Methods The WB-F344 cells were cultured and treated in sodium butyrate.The total protein at 0,2,4,6d during the differentiation was extracted and analyzed by iTRAQ technology coupled with mass spectrometric analysis.Results A total of 67 significant protein spots were found by mass spectrometric analysis,among which,33 spots were up-regulated and 34 spots were down-regulated in WB-F344 cell differentiation.Conclusion This study indicated iTRAQ is a useful technologic method in proteomic study of cell differentiation and the results provide the experimental foundation for elucidating molecular mechanism of WB-F344 cell differentiation. |
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| Keywords: | WB-F344 cells differentiation proteome iTRAQ |
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