| H2O2和NaHS对几种脱毛酶活力的影响 |
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| 引用本文： | 许伟,张宗才,戴红.H2O2和NaHS对几种脱毛酶活力的影响[J].中国皮革,2007,36(7):29-32. |
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| 作者姓名： | 许伟 张宗才 戴红 |
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| 作者单位： | 1. 陕西科技大学资源与环境学院,陕西,西安,710021
2. 四川大学轻纺与食品学院,四川,成都,610065 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 采用福林法和紫外分光光度法分析了几种蛋白酶活力的pH值曲线,并探讨了H2O2及NaHS对它们酶活力变化的影响.结果表明H2O2对NOWOLASE PE酶的活力影响较小,且具有一定的激活作用,而对2709酶、A.S1398酶和Basozym L10酶的影响极大;NaHS对2709酶和A.S1398酶的活力影响较小,且对2709酶的活力具有稳定作用,而对NOWOLASE XL酶、NOWOLASE PE酶和Basozyme L10酶的影响大.此结果为研究H2O2和NaHS辅助蛋白酶脱毛,以解决酶脱毛中小毛不易脱净的缺陷打下基础.
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| 关 键 词： | 酶脱毛 酶活力 分析 硫化物 过氧化氢 |
| 修稿时间： | 2006年1月13日 |
Influence of Peroxide and Sodium Hydrosulfide on Activity of Several Depilatory Enzymes |
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| XU Wei,ZHANG Zong-cai,DAI Hong.Influence of Peroxide and Sodium Hydrosulfide on Activity of Several Depilatory Enzymes[J].China Leather,2007,36(7):29-32. |
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| Authors: | XU Wei ZHANG Zong-cai DAI Hong |
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| Abstract: | Curves of pHvalue of several enzymes were analyzed by FOLINmethod and UltravioletSpectrophotometric method. The influences of peroxide and sodiumhydrosulfide on the enzymes activity were investigated. The results showe that peroxide had little influence on NOWOLASE PE enzyme activity, on the contrary, it has some activation effect on it. While peroxide has great impact on 2709 enzyme, A.S1398 enzyme and BasozymL10 enzyme. However, sodiumhydrosulfide has great impact on NOWO- LASE XL enzyme, NOWOLASE PE, Basozyme L10 enzyme and little impact on 2709 enzyme and A.S1398 enzyme. Further- more, itcould stabilize activity of2709 enzyme. The study is helpful for the investigation on enzymatic unhairingassisted with per- oxide and sodium hydrosulfide to destroy short hair. |
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| Keywords: | enzymatic unhairing enzyme activity analysis sulfide peroxide |
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