MMP-9和TIMP-3在内异症患者子宫内膜中的表达

目的 观察比较基质金属蛋白酶（MMPs）和基质金属蛋白酶抑制物（TIMPs）在异位、在位和正常子宫内膜中的表达，探讨其与子宫内膜异位症（内异症）的关系。方法应用免疫组化LSAB法和KS400图像分析系统，检测MMP-9和TIMP-3在26例卵巢子宫内膜异位囊肿患者的异位子宫内膜和其中15例在位子宫内膜中的表达，分析阳性细胞的分布规律，以20例正常子宫内膜作为对照。结果MMP-9和TIMP-3在正常和异位子宫内膜的腺上皮细胞、基质细胞中均有不同程度的表达。异位子宫内膜组织中MMP-9呈高表达状态，TIMP-3表达减少，与正常子宫内膜和在位内膜相比有显著差异（P〈0．05）；在位子宫内膜与正常子宫内膜MMP-9和TIMP-3的表达无显著差异（P〉0．05）。结论提示异位内膜组织具有更强的侵袭力，其在子宫内膜异位症发病中起重要作用。

Expression of MMP-9 and TIMP-3 in Endometrium from Women with Endometriosis

Objective To evaluate the expression and localization of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs)in the ectopic and eutopic endometrium from women with and without endometriosis. Thereby to determine this role in the pathogenesis of endometriosis. Methods The expression of MMP-9 and TIMP-3 protein on paraffin-embedded tissues was detected by immunohistochemical analysis- LSAB and image analysis.The study included endometriotic tissues (n=26) and matched eutopic endometrium (n=15) of patients with endometriosis and eutopic endometrium of normal menstruating women without endometriosis (n=20). Results The variant expression of MMP-9 and TIMP-3 was detected in both eutopic and ectopic endometrium. Ectopic tissues showed statistically stronger MMP-9 and weaker TIMP-3, compared with the eutopic endometrium from normal and endometriosis patients (P<0.05). However,There was no difference in the expression between the eutopic endometrium of women with endometriosis and the normal controls. Conclusion The results suggest that ectopic endometrium may be more invasive and lead to endometriosis. MMPs and TIMPs play an important role in the pathogenesis of the disease.