促卵泡激素结合片段对卵巢癌细胞株的作用

目的 研究促卵泡激素９ＦＳＨ）结合片段对卵巢癌细胞株的作用了解ＦＳＨ结合片段作为卵巢靶向治疗的导向材料的应用价值。方法 应用不同类型的卵巢癌细胞株ＳＫＯＶ３、ＯＶＣＡＲ、ＡＯ、３ＡＯ作为实验对象,分别加入ＦＳＨ、ＦＳＨ结合片段、ＦＳＨ＝ＦＳＨ结合片段。培养５天后用四甲基偶氮唑蓝（ＭＴＴ）法检测卵巢癌细胞的生长状况。结果 ＦＳＨ对卵巢癌细胞的生长有明显的促进作用,细胞平均增殖率为２８．０％;ＦＳＨ结

Effects of synthetic peptides on ovarian cancer cells

Objective To observethe effectoffollicle stimulating hormone(FSH)synthetic peptides(FSH binding Fragment),FSHand synthetic peptides on the proliferation of human epithelial ovarian cancer cell- Methods Human epithelialovarian cancercellslinesSKOV3 ,OVCAR,AOand 3AOwereincubated with FSH, FSHbindingfragment,FSHandthe bindingfragmentrespectively- The cell proliferation was detected by methyl thiazolyltetrazolium ( MTT) technique- Results The rate of proliferation in the cancer cell was increased apparently asincreasinginthe concentration ofFSHandthe rate ofproliferation average value 28-0% ,and was decreased apparently asincreasinginthe concentration ofthe synthetic peptidesandtherate ofinhibition average value 8-3%- Whenthe cell wasexpressedin FSH,the proliferation wasdecreased apparentlyasincreasinginthe concentration ofthe peptides and the rate ofinhibition average valve 3 .1 % - Conclusions Itis suggested that FSHbindingfragmentcaninhibitthe proliferation ofovarian cancercell- The FSHbindingfragmentcould be used asa binding partofanticancerons complexforovarian cancer-