正交试验法优选温阳活血颗粒的提取工艺

目的:优选温阳活血颗粒的提取工艺。方法:采用HPLC测定芍药苷含量,流动相乙腈-0.1%磷酸水溶液(14∶86),检测波长230 nm;利用HPLC-ELSD测定人参皂苷Rg1,Re,Rb1含量,流动相乙腈(A)-水(B)梯度洗脱(0~35 min,19%A;35~55 min,19%~29%A;55~70 min,29%A;70~100 min,29%~40%A)。以干膏率和有效成分(人参皂苷Rg1,Re,Rb1和芍药苷)总含量的综合评分为评价指标,通过正交试验考察加水量、提取时间和提取次数对温阳活血颗粒提取工艺的影响。结果:最佳提取工艺为加8倍量水回流提取3次,每次1.5 h;干膏率依次为39.65%,人参皂苷Rg1,Re,Rb1和芍药苷提取量分别为3.49,2.79,6.64,218.60 mg。结论:优选的提取工艺科学合理、简便可行,适用于温阳活血颗粒的工业化生产。

Optimization of Extraction Technology for Wenyang Huoxue Granules by Orthogonal Test

Objective: To optimize extraction technology of Wenyang Huoxue granules. Method: HPLC was adopted to determine the content of paeoniflorin with mobile phase of acetonitrile-0.1% phosphoric acid solution (14:86) and detection wavelength at 230 nm;HPLC-ELSD was employed to determine the content of ginsenoside Rg₁, Re, Rb₁with mobile phase of acetonitrile(A)-water(B) for gradient elution (0-35 min, 19%A;35-55 min, 19%-29%A;55-70 min, 29%A;70-100 min, 29%-40%A).With yield of dry extract and total contents of paeoniflorin, ginsenoside Rg₁, ginsenoside Re, ginsenoside Rb₁ as comprehensive evaluation index, orthogonal design was taken for investigating effects of the amount of water, extraction times and time on extraction technology. Result: Optimal extraction conditions were as followings:reflux extracted thrice with 8 times the amount of water for 1.5 hours each time;yield of dry extract was 39.64%, contents of ginsenoside Rg₁, Re, Rb₁ and paeoniflorin were 3.49, 2.79, 6.64, 218.60 mg. Conclusion: This optimized technology is reasonable, simple and suitable for industrial production of Wenyang Huoxue granules.