| 长白山人参的毛细管电泳指纹图谱 |
| |
| 引用本文： | 阎正,王春云,苑若瑶,蔡立鹏,王继坤.长白山人参的毛细管电泳指纹图谱[J].河北大学学报(自然科学版),2009,29(1):55. |
| |
| 作者姓名： | 阎正 王春云 苑若瑶 蔡立鹏 王继坤 |
| |
| 作者单位： | 1.河北大学,化学与环境科学学院,河北,保定,071002； 2.北京中防昊通防伪技术中心,北京,100025 |
| |
| 摘 要： | 建立了长白山人参的高效毛细管电泳(HPCE)指纹图谱.研究了不同的预处理方法、检测波长、分离电压、缓冲体系对人参指纹图谱的影响,并对原产地长白山人参和人工种植人参的指纹图谱进行了比较.采用毛细管区带电泳法测定指纹图谱,电泳条件:75 μm i.d.未涂层石英毛细管(有效长度65 cm),以20 mmol/L的硼砂缓冲体系做背景电解质,分离电压18 kV,检测波长243 nm,气压进样3 s,进样压力8 kPa,温度为室温.
|
| 关 键 词： | 人参 高效毛细管电泳 指纹图谱 长白山 |
Fingerprint Chromatogram of Ginseng by HPCE |
| |
| YAN Zheng,WANG Chun-yun,YUAN Ruo-yao,CAI Li-peng,WANG Ji-kun.Fingerprint Chromatogram of Ginseng by HPCE[J].Journal of Hebei University (Natural Science Edition),2009,29(1):55. |
| |
| Authors: | YAN Zheng WANG Chun-yun YUAN Ruo-yao CAI Li-peng WANG Ji-kun |
| |
| Affiliation: | 1.College of Chemistry and Environmental Science;Hebei University;Baoding 071002;China;2.Zhongfang Haotong Antiforgery Technology Center of Beijing;Beijing 100025;China |
| |
| Abstract: | Establish the high-performance capillary electrophoresis(HPCE) fingerprint chromatogram of Changbaishan ginseng.The effects of different pre-treatment methods,detection wavelength and separate voltage on ginseng fingerprint chromatogram are studied.The fingerprint chromatograms of Changbaishan ginseng and artificial ginseng are compared.Capillary zone electrophoresis is adopted for fingerprint chromatogram measurement.HPCE conditions include:uncoated quartz capillary 75 μm i.d.(available length:65 cm),background electrolyte of 20 mmol/L borax buffer system,separate-voltage 18 kV,wavelength 243 nm,sampling pressure 8 kPa,room temperature. |
| |
| Keywords: | ginseng HPCE fingerprint chromatogram Changbaishan |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《河北大学学报(自然科学版)》浏览原始摘要信息 |
|
点击此处可从《河北大学学报(自然科学版)》下载全文 |
|
