新疆喀什酸石榴皮总多酚有效部位的纯化工艺优选

目的: 优选喀什酸石榴皮总多酚有效部位的纯化工艺。 方法: 以总多酚含量为指标，通过静态吸附-洗脱试验筛选大孔树脂型号，采用单因素试验考察上样液质量浓度、洗脱剂用量、吸附流速等对酸石榴皮总多酚纯化工艺的影响。 结果: 选用HPD500型大孔吸附树脂，最佳纯化工艺为上样液生药质量浓度20.8 g·L^-1，上样液pH 2，上样量8 BV，吸附速度2 BV·h^-1，吸附时间12 h，加水7 BV洗脱杂质，用50%乙醇8 BV洗脱，收集乙醇洗脱液;总多酚质量分数由纯化前的24%增加至66.93%，浸膏得率34.80%。 结论: HPD500型大孔树脂适用于酸石榴皮总多酚的纯化，优选的工艺条件简便易行、高效低毒。

Optimization of Purification Technology for Total Polyphenols from Acid Granti Pericarpium in Kashi

Objective: To optimize purification process of total polyphenols from acid Granti Pericarpium in Kashi. Method: With the content of total polyphenols as index,type of macroporous resin was screened by static adsorption-elution test,single factor tests were adopted to optimize purification process of total polyphenols by taking sample solution concentration,eluent consumption,adsorption speed and others as factors. Result: HPD500 macroporous resin was selected,optimum purification process was as follows:the crude drug concentration of sample solution 20.8 g· L^-1,pH of sample solution 2,sample volume 8 BV,adsorption rate 2 BV· h^-1,adsorption time 12 h,eluted impurities with 7 BV of distilled water and 8 BV of 50% ethanol,collected ethanol eluate;Purity of total polyphenols was up to 66.93% from 24% before purification,yield of extract was 34.80%. Conclusion: HPD500 macroporous resin was suitable for purification of total polyphenols from acid Granti Pericarpium,optimized process conditions were simple and feasible with high efficiency and low toxicity.