双苓扶正抗癌胶囊对小鼠肝癌的影响

目的：研究双苓扶正抗癌胶囊对小鼠肝癌的抑制作用及作用机制。方法：建立小鼠肝癌（Heps）实体瘤及腹水癌模型，观察抑瘤率和生长延长率，并用流式细胞术（FCM）检测小鼠肝癌实体瘤的细胞周期及癌细胞凋亡率。结果：双苓扶正抗癌胶囊在15g和30g/kg剂量下，对小鼠肝癌实体瘤有显著抑制作用，也能延长荷腹水型肝癌小鼠的生存期；还可引起小鼠肝癌瘤细胞S期及G2-M期细胞减少，G0-G1期细胞及凋亡细胞增加。结论：双苓扶正抗癌胶囊对小鼠移植性肝癌具有抑制作用，其作用机制可能与抑制细胞周期中S期DNA合成、干扰细胞的分裂增殖速度及诱导癌细胞凋亡有关。

Experimental Study of Shuang Ling Fuzheng Kangai Capsule(SLFKC) on Inhibiting Heps of Mouse

Objective:To study the inhibitory effect and mechanism in Heps of mouse with Shuang Ling Fuzheng Kangai Capsule(SLFKC). Method:Mouse model of the solid tumor and ascitic cancer were established.The inhibitory rate of solid tumor and the life prolongation rate of ascites cancer were observed.The cell cycle and apoptosis of the solid tumor cells were measured by Flow Cytometry(FCM). Results:SLFKC(15 and 30g.kg -1 .d -1 ) had significant restraning effect on Heps of mouse and the life of ascites cancer bearing mice was also prolonged. SLFKC could decrease the number of cells in S and G\-2\|M phase and increase the number of cells in G 0 G 1 phase and the number of apoptosis cells. Conclusion:SLFKC has inhibiting effect on Heps of mouse. The anticancer mechanism was probably related to the synthesis of DNA during S phase,the interference with the speed of cell proliferdtion,and inducement of the apoptosis of cancerous cells.