In vivo MR imaging of plaque disruption and thrombus formation in an atherosclerotic rabbit model |
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Authors: | Xiaohai Ma Quanming Zhao Lei Zhao Jianfeng Shang Tingting Feng Conghe Zeng Zhaoqi Zhang |
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Affiliation: | (1) Department of Radiology, Beijing Anzhen Hospital, Capital Medical University, 2 Anzhen Rd., Chaoyang Dist., Beijing, China;(2) Department of Cardiology, Beijing Anzhen Hospital, Capital Medical University, 2 Anzhen Rd., Chaoyang Dist., Beijing, China;(3) Department of Pathology, Beijing Anzhen Hospital, Capital Medical University, 2 Anzhen Rd., Chaoyang Dist., Beijing, China; |
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Abstract: | Our aim is to introduce an atherosclerotic rabbit model for inducing atherosclerosis lesions in rabbits, and to validate the
model in vivo with 3T high resolution magnetic resonance imaging of the thrombosis followed a pharmacologically triggered
plaque disruption. Twenty male New Zealand White rabbits were randomly allocated into an experimental group (n = 16) and a
control group (n = 4). The aortic wall injuries were induced by an intravascular balloon in the experimental group rabbits
after feeding them with a high cholesterol diet for 2 weeks. The pharmacological triggering with Russell’s viper venom and
histamine was performed after totally 16 weeks of intermittent cholesterol feeding. All of the animals underwent both the
pre-trigger and post-trigger MR examinations including TOF, T1WI, T2WI and post contrast T1WI. Euthanasia was performed in
all rabbits; gross anatomy and histological specimen of aorta were obtained. MR images were analyzed and compared with histological
results. Compared with the control group rabbits, the aorta of the experimental group rabbits in the pre-triggered MR images
showed an increased vessel wall thickening, luminal narrowing, and vessel wall enhancement. Fourteen rabbits survived the
triggering, and 8 of them developed thrombosis (58.1%). No thrombus was found in the control group. The accuracy of the multi-sequences
MR including TOF, T1WI, T2WI and post contrast T1WI was 87.1% (27/31) for detecting thrombus. MR data significantly correlated
with the histopathology data for both thrombus length (r = 0.94, P < 0.01) and thrombus location (r = 0.85, P < 0.01), respectively. The study demonstrated that MR reliably determined the plaque disruption and thrombus formation in
the atherosclerotic rabbit model. |
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