| 高效液相色谱法测定银栀口服液中绿原酸的含量 |
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| 引用本文： | 李翔,刘皈阳,马建丽,周亮,高磊.高效液相色谱法测定银栀口服液中绿原酸的含量[J].实用药物与临床,2013,16(1):47-48. |
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| 作者姓名： | 李翔 刘皈阳 马建丽 周亮 高磊 |
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| 作者单位： | 解放军总医院第一附属医院药剂药理科 |
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| 摘 要： | 目的建立测定银栀口服液中绿原酸含量的高效液相色谱方法。方法采用Agilent EclipseXDB-C18(4.6 mm×150 mm,5μm)色谱柱,流动相为甲醇-0.5%冰醋酸溶液(20∶80),流速:1.0 mL/min,检测波长:327 nm,柱温:30℃,进样量:10μL。结果绿原酸浓度在2.184～109.2μg/mL范围内与峰面积线性关系良好,平均回收率为98.65%,RSD为1.48%(n=6)。结论本方法操作简便,结果准确,专属性强,灵敏度高,可用于银栀口服液中绿原酸的含量测定。
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| 关 键 词： | 银栀口服液 绿原酸 高效液相色谱法 含量测定 |
Determination of chlorogenic acid in yinzhi oral solution by HPLC |
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| LI Xiang,LIU Gui-yang,MA Jian-li,ZHOU Liang,GAO Lei.Determination of chlorogenic acid in yinzhi oral solution by HPLC[J].Practical Pharmacy and Clinical Remedies,2013,16(1):47-48. |
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| Authors: | LI Xiang LIU Gui-yang MA Jian-li ZHOU Liang GAO Lei |
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| Affiliation: | (Department of Pharmacy,First Affiliated Hospital of PLA General Hospital,Beijing 100048,China) |
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| Abstract: | Objective To establish an HPLC method for determination of chlorogenic acid in yinzhi oral solution.Methods The Agilent Eclipse XDB-C18(4.6 mm×150 mm,5 μm)was used,the mobile phase consisted of methanol-0.5% acetic acid(20∶ 80),the flow rate was 1.0 mL/min,the detection wavelength was 327 nm,the column temperature was 30 ℃,the inject volume was 10 μL.Results A good linear correlation of chlorogenic acid was observed within the range of 2.184~109.2 μg/mL.The average recovery rate was 98.65% with RSD of 1.48%(n=6).Conclusion The method is rapid,simple and accurate,it can be used for the determination of chlorogenic acid in yinzhi oral solution. |
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| Keywords: | Yinzhi oral solution Chlorogenic acid HPLC Content determination |
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