醛固酮促进心肌成纤维细胞增殖的实验研究

目的：研究醛固酮对培养心肌成纤维细胞增殖的影响。方法：培养新西兰白兔心肌成纤维细胞，将第三代心肌成纤维细胞分为醛固酮组（不同浓度）、醛固酮＋螺内酯组和对照组，心肌成纤维细胞的代谢活性与DNA合成分别用WST-1试剂和5′溴脱氧尿嘧啶核苷（BrdU）酶联免疫吸附试验（ELISA）进行测定。结果：与对照组比较，不同浓度醛固酮作用后，心肌成纤维细胞的WST-1与BrdU的OD值均增加，并呈浓度依赖性（P〈0．05～〈0．01）；丽螺内酯＋醛固酮组的WST-1与BrdU的OD值明显低于醛固酮组（P〈0．01）。结论：在体外，醛固酮能诱导心肌成纤维细胞增殖，而螺内酯抑制其作用。

Effect of aldosterone on the proliferation of cultured myocardiac fibroblasts

Abstract： Objective： To investigate the effect of aldosterone on the proliferation of cultured myocardiac fibroblasts in vitro. Methods.. Myocardiac myofibroblasts derived from the heart of New Zealand rabbit were cultured. The 3^rd passage of cells were divided into the following groups： aldosterone groups （in different concentration）, aldosternoe＋spironolactone group and control group. Both of cells metabolic activity and DNA synthesis were evaluated with WST- 1 cleavage and BrdU incorporation assay （ELISA）. Results： Compared with the control group, the OD values of both WST--1 and BrdU assay were significantly increased in a manner of dose-dependent pattern in aldosterone groups （P 〈0. 05）. However, the OD of WST--1 and BrdU in aldosternoe＋spironolactone group was lower than that in aldosterone groups （P〈0.01）. Conclusion： Aldosterone can promotes the proliferation of cultured myocardiac fibroblasts in vitro. The effect of aldosterone can be blocked by spironolactone.