超高效液相色谱同时测定食品中4种工业染料

目的建立同时测定食品中酸性橙、碱性橙、碱性嫩黄、罗丹明B工业染料的方法。方法样品用水-5%氨水乙腈-正己烷混合溶剂提取净化,采用ZORBAX Extend C18(2.1 mm i.d.×50 mm,1.8μm)分离,以乙腈—乙酸铵缓冲液梯度洗脱,流速为0.5 ml/min,二极管阵列检测器检测酸性橙(λ=485 nm)、碱性橙及碱性嫩黄(λ=435nm)、罗丹明B(λ=548 nm)。结果 4种组分在0.1～10.0 mg/L范围内有良好的线性关系(r>0.999),检测限为0.006 4～0.019 mg/kg。在番茄沙司、腊肠、辣椒油3种不同食品基质中平均加标回收率为70.0%～102.7%,相对标准偏差为0.5%～3.1%。结论方法快速,简单,可应用于食品中酸性橙、碱性橙、碱性嫩黄、罗丹明B 4种工业染料的同时检测。

Simultaneous determination of four industrial dyes in foods by ultra high performance liquid chromatography

Objective To establish a method for the simultaneous determination of Orange 2,Aurnine O,Chrysoidine G and Rhodamine B in foods by Ultra High Performance Liquid Chromatography(UHPLC).Methods Samples were extracted and cleaned up with water-5% ammonia acetonitrile-n-Hexane mixture solvents.UHPLC separation was achieved by using ZORBAX Extend C18(2.1 mm i.d.×50 mm,1.8 μm) with an mobile phase consisting of acetonitrile and acetate buffer,and gradient program was used at a flow rate of 0.5 ml/min.The detection wavelength was set at 485nm for Orange 2,435 nm for aurnine O and chrysoidine G,while 548 nm for rhodamine B.Results There was a good linear relationship(r>0.999) in the range of 0.1-10 mg/L for these four dyes.The limits of detection(LOD) were from 0.006 4 to 0.019 mg/kg respectively.The average recoveries for all four dyes in tomato sauce,sausage and chili powder were ranged 70.0%-102.7%,the relative standard deviations(RSDs) were 0.5%-3.1%.Conclusion The method is rapid,simple and could be applied for the simultaneous determination of Orange 2,Aurnine O,Chrysoidine G and Rhodamine B in different kinds of foods.