芦荟大黄素对斑马鱼胚胎发育及运动行为学的毒性研究

以斑马鱼胚胎为模型，研究芦荟大黄素对胚胎发育和行为学的影响。结果显示，1.0 μg/mL及以上浓度的芦荟大黄素对斑马鱼胚胎有致死作用，随着浓度的增加和作用时间延长而增强。芦荟大黄素对斑马鱼胚胎的孵化过程有阻滞作用，1.0 μg/mL及以上浓度的芦荟大黄素使斑马鱼胚胎出现了卵黄延伸异常、发育迟缓和身体弯曲等畸形现象，但是对斑马鱼仔鱼的运动行为学无明显影响。本实验结果提示，临床应用芦荟大黄素治疗如孕妇这类特殊病人时，应充分考虑药物的剂量和用药时间。

Toxicity of aloe-emodine to embryos growth and behavior of zebrafish

We addressed the impact of aloe emodine on embryos growth and behavior of zebrafish with zebrafish embryo as a model. Experimental results show that aloe emodineof more than 1.0 μg/mL concentration has lethal effect to zebrafish embryos. This effect is strengthened with the increase of concentration and action time. Aloe emodine has obstruction effect to the incubation of zebrafish embryos. Aloe emodine of more than 1.0 μg/mL concentration makes zebrafish embryos to exhibit such malformation as yolk abnormal extension, growth retardation and body bending. However, Aloe emodine has no obvious effect on the behavior of zebrafish larvae. Experimental results demonstrate that we should fully consider aloe emodine dosage and medication time when we clinically apply aloe emodine to the treatment for such special patients as pregnant women.