粘虫幼虫血淋巴脂蛋白的分离鉴定及理化特性分析

通过利用NaBr密度梯度超速离心对粘虫Mythimna separata幼虫的血脂蛋白进行了分离及理化特性分析。查明了幼虫血淋巴内主要是高密度脂蛋白(HDLp)，而低密度脂蛋白(LDLp)的含量极低。这两种脂蛋白的密度分别为HDlp 1.123g/Ml，LDLp 1.059g/mL。HDLp和LDLp蛋白含量、脂类、氨基酸的种类测定结果表明HDLp，LDLp的蛋白含量分别是25.2μg/μL和11μg/μg。HDLp水解后可获得16种氨基酸。LDLp可获得15种。氨基酸的种类比较一致，其含量变化幅度也不大。HDLp脂类部分占最多的是二酰基甘油酯(DC)和磷脂(PC)。其余的脂类依次是碳氢化合物(HC)和胆固醇?。LDLp主要是磷脂和碳氢化合物。昆虫脂蛋白的主要中性甘油酯是二酰基甘油酯。

ULTRACENTRIFUGAL ANALYSIS AND BIOCHEMICAL PROPERTIES OF LIPOPROTEIN IN HEMOLYMPH OF LARVAL ARMYWORM, MYTHIMNA SEPARATA

Two kinds of lipoproteins, low density lipoproteins (LDLp, d= 1. 059)and high density lipoproteins (HDLp, d=1.123) were isolated from the larval hemolymph of Mythimna separata by ultracentrifugal analysis. LDLp was obtained as a single protein band on poly-acrylamide gel electrophoresis, however, HDLp could besepartated into several protein bands by electrophoresis. Biochemical properties of HDLp and LDLp have been analyzed. Protein contents of HDLp and LDLp were 25.2 /μg/μL and 11/μg/μL respectively. Composition of amino acids of HDLp and LDLp were analyzed by amino acids.analyser. The amino acid composition was compared with thatin other insects. The major lipid component of lipoproteins from insects was found to be diglycerides (DO).