CEA-rV负荷DC诱导CIK对CEA+肿瘤细胞的杀伤研究

  目的 用CEA-rV负荷脐血来源树突状细胞（DC）后，再诱导CEA抗原特异的细胞因子诱导的杀伤细胞，研究其对CEA阳性肿瘤细胞株杀伤活性的作用。方法 用淋巴细胞分离液分离脐带血单核细胞，分别诱导DC、CIK细胞及CEA-rV负载DC后，再与CIK细胞混合培养诱导CEA特异的细胞毒性T淋巴细胞。分别用以上不同类型细胞杀伤CEA+和CEA-的肿瘤细胞株。结果 CEA-rV诱导的特异性CIK对CEA阳性肿瘤细胞的杀伤活性，Lovo为58.2 %、A549为62.4 %，较之DC-CIK组对CEA阳性肿瘤细胞Lovo为44.8 % 、A549为50.2 %，具有更高的杀伤活性，差异有统计学意义（P＜0.05）。CEA-rV诱导的特异性CIK对CEA阴性肿瘤细胞的杀伤活性K562为79.7 %、Bel7402为70.1 %，DC-CIK组对CEA阴性肿瘤细胞K562为78.7 %、Bel7402为67.8 % ，两组间无明显区别，差异无统计学意义（P＞0.05）。用CEA-rV诱导的特异性CIK对CEA阳性肿瘤细胞Lovo杀伤提高了89.4 %，A549提高了83.1 %；而对于CEA阴性肿瘤细胞K562杀伤提高了32.5 %，Bel7402提高了21.7 %，两组间差异有统计学意义（P＜0.05）。结论 负载CEA-rV基因重组痘苗的DCs（CEA-rV-DCs）诱导的细胞毒性T淋巴细胞（CEA-rV-DCs-CIK）对CEA阳性肿瘤细胞有特异性杀伤能力。

The anti-tumor study induced by CIK cocultured with dendritic cell loaded with CEA recombinant virus

Objective To survey the anti-CEA-positive tumor with special immune response enhanced by cytokine induced killer(CIK) cells cocultured with umbilical cord blood dendritic cells(DCs) loaded with CEA recombinant vaccinia virus(CEA-rV). Methods Freshly isolated umbilical blood mononuclear cells(UBMCs) were cultivated. Suspension cells and attached cells were used to induce CIK cells and DCs separately. DCs were loaded with CEA-rV, i.e. CEA-rV+DC. CIK cells were cocultured with CEA-rV-DCs, i.e. CEA-rV+DC+CIK. The killing activity of each effector cell, including UBMCs, CIK, DC+CIK and CEA-rV+DC+CIK, was measured respectively by MTT reduction assay. There are CEA positive tumor cell and CEA negative tumor cell in the target cell groups. Results The killing to Lovo and A549 activity of CEA-rV+DC+CIK was 58.2 %, 62.4 %, which was higher than that of DC+CIK about 44.8 % and 50.2 %. Significant difference had also been shown in those groups in statistics. However there was no significant difference in statistics to CEA negative cell lines Bel7402 and K562, the CEA-rV+DC+CIK groups 79.7 %, 70.1 % and the DC+CIK groups 78.7 %, 67.8 %. Furthermore, the raise of killing activity to Lovo and A549 of CEA-rV+DC+CIK was 89.4 %, 83.1 %, the raise of killing activity to Bel7402 and K562 of CEA-rV+DC+CIK was 32.5 %, 21.7 %. The raise of killing activity to CEA positive cell lines is more higher than that of the CEA negative groups. There is significance difference in statistics. Conclusions CEA-rV+DCs+CIK showed a special killing activity to CEA positive tumor cell lines.