慢性胃炎胃黏膜细胞DNA含量和增殖活性的流式细胞仪检测

背景慢性萎缩性胃炎是一种胃癌癌前状态,研究表明正常胃黏膜、癌前病变和胃癌细胞的DNA含量随病变的进展而逐渐增高。目的应用流式细胞仪检测慢性胃炎胃黏膜细胞的DNA含量和增殖活性,探讨两者在慢性胃炎发生、发展过程中的临床意义。方法选取90例经胃镜检查诊断为慢性胃炎者的胃黏膜活检标本,制备单细胞悬液,应用流式细胞仪进行细胞DNA含量和增殖活性检测。结果所有慢性胃炎胃黏膜细胞的DNA倍体类型均为二倍体,但慢性萎缩性胃炎和慢性萎缩性胃炎伴肠化生胃黏膜细胞的增殖指数(PI)较慢性非萎缩性胃炎显著增高(P<0.05)。除慢性非萎缩性胃炎外,其余慢性胃炎组幽门螺杆菌(H.pylori)阳性患者胃黏膜细胞的PI值均较阴性患者显著增高(P<0.05)。结论慢性萎缩性胃炎和H.pylori阳性慢性胃炎胃黏膜细胞的增殖活性显著增高。应用流式细胞仪检测胃黏膜细胞的DNA含量和增殖活性,也许能成为胃癌癌前状态和癌前病变病理诊断的参考指标。

Flow Cytometry Analysis of DNA Content and Cell Proliferation of Gastric Mucosal Cells in Chronic Gastritis

Background: Chronic atrophic gastritis is defined as a precancerous condition of gastric cancer. It has been reported that the cell DNA content increases from normal gastric mucosa to precancerous lesions and gastric cancer. Aims: To analyze the DNA content and cell proliferation of gastric mucosal cells in chronic gastritis by using flow cytometry, and to investigate their clinical significances in the development of chronic gastritis. Methods: Gastric mucosal biopsy specimens were obtained from 90 chronic gastritis patients diagnosed by gastroscopy. Single cell suspension was prepared to analyze the DNA content and cell proliferation with flow cytometry. Results: The diploid DNA was found in all gastric mucosal cells of chronic gastritis, but the proliferation index (PI) of gastric mucosal cells in chronic atrophic gastritis and chronic atrophic gastritis with intestinal metaplasia increased more significantly than that in chronic non-atrophic gastritis (P<0.05). Except for the chronic non-atrophic gastritis, the PI of gastric mucosal cells in Helicobacter pylori (H. pylori)-positive chronic gastritis patients increased also significantly more than that in H. pylori-negative ones (P<0.05). Conclusions: The proliferation activity of gastric mucosal cells increases in chronic atrophic gastritis and H. pylori-positive chronic gastritis. The DNA content and cell proliferation of gastric mucosal cells analyzed by flow cyto-metry may be served as a reference in the pathological diagnosis of precancerous condition and precancerous lesion of gastric cancer.