比较两种不同来源间充质干细胞的生物学特性及多向分化潜能

该文旨在比较人脂肪间充质干细胞(hADSCs)和脐带间充质干细胞(hUMSCs)的生物学特性,并鉴定其多向分化潜能。体外扩增培养hADSCs和hUMSCs,绘制细胞生长曲线并计算群体倍增时间,通过细胞集落实验比较两种细胞的增殖能力,流式细胞仪和RT-PCR方法分别检测细胞表面抗原和多能性相关基因的表达,采用成脂、成骨诱导分化试剂盒比较两种细胞的分化潜能。hADSCs和hUMSCs表达CD34、CD44、CD45、CD105的比例分别为2.7%vs 6.2%、92.3%vs 93.4%、1.3%vs 3.1%、99.4%vs 98.0%,均表达Oct4和Nanog基因;生长曲线均呈"S"型,两种细胞的群体倍增时间差异不显著(P>0.05);随着代数的增加,两种细胞的增殖能力均变弱,但P7的hADSCs的增殖能力要显著优于hUMSCs(P<0.05);经油红O、茜素红染色及RT-PCR和细胞免疫荧光方法检测特异基因的表达,表明两种细胞均具备成脂、成骨分化的能力,hADSCs的成脂能力优于hUMSCs,但两种间充质干细胞的成骨分化能力没有显著性差异。hADSCs和hUMSCs具有相似的生物学特性,但hADSCs可能具备更强的增殖能力和成脂分化潜能。

Comparison of Biological Characteristics and Multiple Differentiation Potential of Mesenchymal Stem Cells from Different Sources

This study aimed to compare the biological characteristics and differentiation potential of hADSCs(human adipose-derived mesenchymal stem cells)and hUMSCs(human umbilical cord mesenchymal stem cells).hADSCs and hUMSCs were cultured in vitro.Cell proliferation was characterized by construction of growth curves and calculation of the PDT(population doubling time).We also compared the cells’proliferative ability by colony forming assays,determined the cell surface antigens by flow cytometry,and tested the pluripotency-related genes by RT-PCR.Differentiation potential was compared by adipogenic,osteogenic induction and differentiation kits.The ratios of CD34,CD44,CD45,CD105 expressed in hADSCs versus hUMSCs were 2.7%vs 6.2%,92.3%vs 93.4%,1.3%vs 3.1%,and 99.4%vs 98.0%,respectively.All cells expressed Oct4 and Nanog and presented an S-shaped growth curve.There was no significant difference in PDT between the two cell types(P>0.05).The proliferative ability of both cell types declined progressively with passaging,but the proliferative ability of hADSCs at passage 7 was significantly higher than hUMSCs(P<0.05).Results of oil red O,alizarin red staining,RT-PCR and cell immunofluorescence suggested that both cell types had the potential to differentiate into adipocytes and osteocytes.However,hADSCs had stronger adipogenic ability than hUMSCs,but there was no significant difference in osteogenic ability between the two cell types.In summary,while hADSCs and hUMSCs share key biological features,hADSCs seem to have stronger proliferative and adipogenic abilities.