| SDF 1α对大鼠骨髓源性内皮祖细胞克隆形成能力及增殖的影响 |
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| 引用本文： | 夏艺萍,危当恒,童中艺,吕运成,姚峰,周晓峰,田永凤,姜志胜,王佐.SDF 1α对大鼠骨髓源性内皮祖细胞克隆形成能力及增殖的影响[J].中国实用医药,2008,3(21):3-5. |
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| 作者姓名： | 夏艺萍 危当恒 童中艺 吕运成 姚峰 周晓峰 田永凤 姜志胜 王佐 |
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| 作者单位： | 湖南省衡阳市南华大学心血管病研究所/动脉硬化学湖南省重点实验室/南华大学护理学院,421001 |
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| 基金项目： | 中国博士后科学基金，湖南省自然科学基金，湖南省教育厅科研项目，湖南省自然科学基金 |
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| 摘 要： | 目的观察基质细胞衍生因子-1α（SDF-1α）对体外培养的大鼠骨髓源性内皮祖细胞（EPCs）动员和增殖的影响。方法微孔法获取大鼠骨髓EPCs并采用荧光显微镜和流式细胞术鉴定EPCs特异性标记物。不同浓度SDF-1α处理EPCs后,采用细胞培养、MTT检测EPCs的克隆形成、细胞增殖。结果通过MTT法检测,对照组与（1、10、100μg/L）SDF-1α处理组的OD值分别为0.311±0.054、0.587±0.072、0.813±0.056、1.029±0.078,通过统计学方法分析,对照组与SDF-1α处理组比较差异均有统计学意义（n=5,P〈0.05）;100μg/LSDF-1α处理组次级内皮祖细胞集落单位数目是对照组近3倍（4.67±1.577比14.33±3.055,n=5,P〈0.01）。结论SDF-1α剂量依赖性地促进EPCs增殖,并显著增强EPCs克隆形成能力。
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| 关 键 词： | 基质细胞衍生因子-1α 内皮祖细胞 增殖 |
Effects of stromal cell-derived factor-1α on proliferation and clone formation of rat bone marrow derived endothelial rrogenitor Cells |
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| XIA Yi-ping,WEI Dang-heng,TONG Zhong-yi,et al..Effects of stromal cell-derived factor-1α on proliferation and clone formation of rat bone marrow derived endothelial rrogenitor Cells[J].China Practical Medical,2008,3(21):3-5. |
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| Authors: | XIA Yi-ping WEI Dang-heng TONG Zhong-yi |
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| Affiliation: | XIA Yi-ping,WEI Dang-heng,TONG Zhong-yi,et al.Insititue of Cardiovascular Disease,Key Lab for Arteriosclerology of Hunan Province,University of Nanhua,Hengyang 421001,China |
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| Abstract: | Objective To investigate the role of stromal cell-derived factor-1α （SDF-1α）to proliferation and clone formation on endothelial progenitor cells（EPC）. Methods Bone marrow derived EPC were indfined by immunofluorescence staining and flow cytometry. Treated with different concentrations of SDF-1α, the property of proliferation was detected by MMT and clone formation was detected with optical microscopy. Results SDF-1α promoted EPC proliferation and clone formation with concentration-dependent manner. Conclusion SDF-1α can improve proliferation of EPC. |
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| Keywords: | Stromal Cell-Derived Factor-1α Endothelial Progenitor Cells Proliferation |
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