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化学调节剂诱导转Bt基因棉花杀虫蛋白和主要抗虫次生物的变化
引用本文:张永军,郭予元,吴孔明,王武刚.化学调节剂诱导转Bt基因棉花杀虫蛋白和主要抗虫次生物的变化[J].棉花学报,2002,14(3):131-133.
作者姓名:张永军  郭予元  吴孔明  王武刚
作者单位:中国农业科学院植物保护研究所,北京,100094
基金项目:国家重点基础研究发展规划项目 (G2 0 0 0 0 1 62 0 8),国家自然科学基金资助项目 (3 9770 498)
摘    要:初步探讨了水杨酸和缩节胺处理转 Bt基因棉花后顶端嫩叶中 Bt杀虫蛋白和主要抗虫次生物发生的变化。结果发现 ,水杨酸和缩节胺处理不能明显影响转 Bt基因棉花顶端嫩叶中 Bt杀虫蛋白的表达量 ;适宜浓度的水杨酸和缩节胺处理能够明显提高转 Bt基因棉花顶端嫩叶中缩合单宁和芸香苷的含量 ;适宜浓度的水杨酸处理还能够明显提高转 Bt基因棉花顶端嫩叶中总抗虫萜烯类物质的含量 ;而缩节胺处理对转 Bt基因棉花顶端嫩叶中总抗虫萜烯类物质的含量影响不大。水杨酸和缩节胺能够在一定程度诱导棉花抗虫次生物的表达

关 键 词:转基因棉  植物生长调节剂  杀虫蛋白  次生代谢物
文章编号:1000-7807(2002)03-0131-03
修稿时间:2001年7月31日

Changes of Bt-ICP and Main Secondary Resistant Metabolites in Bt Transgenic Cotton after being Induced by Chemical Regulators
ZHANG Yong jun,GUO Yu yuan,WU Kong ming,WANG Wu gang.Changes of Bt-ICP and Main Secondary Resistant Metabolites in Bt Transgenic Cotton after being Induced by Chemical Regulators[J].Cotton Science,2002,14(3):131-133.
Authors:ZHANG Yong jun  GUO Yu yuan  WU Kong ming  WANG Wu gang
Abstract:The effects of salicylic acid (SA) and mepiquat chloride (PIX) on the contents of Bt ICP and the secondary resistant metabolites in Bt transgenic cotton terminal leaves was investigated. The primary results showed that SA and PIX had no significant effect on Bt ICP expression in Bt transgenic cotton terminal leaves. Proper concentration of SA and PIX applied on cotton could induce plant resistance by way of increasing the relative condensed tannin and rutin content, and also, proper concentration of SA applied on cotton was able to obviously enhanced the content of total terpenoids in cotton terminal leaves. However, PIX has no significant effect on total terpenoids of cotton terminal leaves. In general, Secondary resistant metabolites could be induced by applied SA and PIX to some degree.
Keywords:Bt transgenic cotton  plant growing regulators  Bt  ICP  Secondary resistant metabolites
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