| 高功率微波对原代培养心肌细胞的影响 |
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| 引用本文： | 邓桦,王德文,曹晓哲,谷庆阳,刘杰,赵梅兰,彭瑞云,董波,王晓民.高功率微波对原代培养心肌细胞的影响[J].军事医学科学院院刊,2003,27(2):121-122,F003. |
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| 作者姓名： | 邓桦 王德文 曹晓哲 谷庆阳 刘杰 赵梅兰 彭瑞云 董波 王晓民 |
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| 作者单位： | 军事医学科学院放射医学研究所,北京,100850 |
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| 基金项目： | 全军医药卫生指令性课题 (0 1L0 2 3 ) |
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| 摘 要： | 目的：观察高功率微波(high Power microwave，HPM)对心肌细胞的影响及细胞内钙高于浓度的变化，探讨HPM对心肌细胞损伤的发生机制。方法：以功率密度950mW／cm^2的HPM辐照心肌细胞后，用荧光染料Fluo—3／AM负载，于激光扫描共聚焦显微镜下观察心肌细胞形态和Ca^2 ]i的变化；用FITC—膜联蛋白(annexin)V／PI双染色法，于流式细胞仪检测心肌细胞的坏死和凋亡。结果：HPM辐照后，细胞内钙高于浓度明显降低，与对照组相比差异显著(P＜0．01)。心肌细胞发生坏死和凋亡，与对照组相比差异显著(P＜0.01)。结论：HPM辐照可引起心肌细胞Ca^2 ]i明显降低，提示HPM辐照可引起心肌细胞膜的损伤，从而造成Ca^2 ]i的大量漏出和细胞的死亡。
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| 关 键 词： | 微波 心肌细胞 细胞凋亡 钙离子 激光扫描共聚焦显微镜 |
| 文章编号： | 1000-5501(2003)02-0121-03 |
Influence of high power microwave on cultured myocardial cells |
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| DENG Hua,WANG De_Wen,CAO Xiao_Zhe,GU Qing_Yang,LIU Jie,ZHAO Mei_Lan,PENG Rui_Yun,Dong Bo,WANG Xiao_Min.Influence of high power microwave on cultured myocardial cells[J].Bulletin of the Academy of Military Medical Sciences,2003,27(2):121-122,F003. |
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| Authors: | DENG Hua WANG De_Wen CAO Xiao_Zhe GU Qing_Yang LIU Jie ZHAO Mei_Lan PENG Rui_Yun Dong Bo WANG Xiao_Min |
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| Abstract: | Objective:To study the damage mechanism of myocardial cells irradiated by HPM, and to observe the injury and changes in intracellular calcium concentration of myocardial cells.Methods:Myocardial cells were irradiated by HPM at power density 950?mW/cm 2, then loaded with fluo_3/AM fluorescence stain.TheCa 2+ ]i was examined by laser scanning confocal microscope(LSCM). Cellular necrosis and apoptosis were examined by flow cytometer.Results:Myocardial cell necrosis and apoptosis were found, and Ca 2+ ]i was decreased significantly ( P <0.01)in myocardial cells irradiated by HPM.Conclusions:The results inferred that HPM could injure myocardial cell membrane,decrease Ca 2+ ]i and induce the death of myocardial cells. |
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| Keywords: | microwaves myocardial cell Ca 2+ laser scanning confocal microscope cell death |
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